Please use this identifier to cite or link to this item:
http://dx.doi.org/10.25673/109847
Title: | Protocol to determine the subcellular localization of protein interactions in murine keratinocytes |
Author(s): | Müller, Lisa![]() |
Issue Date: | 2023 |
Type: | Article |
Language: | English |
Abstract: | Protein activities and interactions are determined by their subcellular localization. Elucidating the network of protein-protein interactions at a spatial resolution is essential for understanding the complexity of protein functions, their regulation, and cellular processes. Here, we present a protocol to determine the subcellular localization of protein interactions in non-transformed murine keratinocytes. We describe steps for nucleus/cytoplasm fractionation, immunoprecipitation from these fractions, and immunoblotting. We then detail binding quantification. |
URI: | https://opendata.uni-halle.de//handle/1981185920/111802 http://dx.doi.org/10.25673/109847 |
Open Access: | ![]() |
License: | ![]() |
Journal Title: | STAR Protocols |
Publisher: | Cell Press |
Publisher Place: | Cambridge, MA |
Volume: | 4 |
Issue: | 2 |
Original Publication: | 10.1016/j.xpro.2023.102309 |
Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
File | Description | Size | Format | |
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1-s2.0-S2666166723002769-main.pdf | 4.72 MB | Adobe PDF | ![]() View/Open |