Please use this identifier to cite or link to this item:
http://dx.doi.org/10.25673/110770
Title: | En route to dynamic life processes by SNARE-mediated fusion of polymer and hybrid membranes |
Author(s): | Otrin, Lado![]() Witkowska, Agata Otrin, Nika ![]() Zhao, Ziliang ![]() Lira, Rafael B. Kyrilism, Fotis L. Hamdi, Farzad Ivanov, Ivan Lipowsky, Reinhard ![]() Kastritis, Panagiotis L. ![]() Dimova, Rumiana ![]() Sundmacher, Kai ![]() Jahn, Reinhard ![]() Vidaković-Koch, Tanja ![]() |
Issue Date: | 2021 |
Type: | Article |
Language: | English |
Abstract: | A variety of artificial cells springs from the functionalization of liposomes with proteins. However, these models suffer from low durability without repair and replenishment mechanisms, which can be partly addressed by replacing the lipids with polymers. Yet natural membranes are also dynamically remodeled in multiple cellular processes. Here, we show that synthetic amphiphile membranes also undergo fusion, mediated by the protein machinery for synaptic secretion. We integrated fusogenic SNAREs in polymer and hybrid vesicles and observed efficient membrane and content mixing. We determined bending rigidity and pore edge tension as key parameters for fusion and described its plausible progression through cryo-EM snapshots. These findings demonstrate that dynamic membrane phenomena can be reconstituted in synthetic materials, thereby providing new tools for the assembly of synthetic protocells. |
URI: | https://opendata.uni-halle.de//handle/1981185920/112725 http://dx.doi.org/10.25673/110770 |
Open Access: | ![]() |
License: | ![]() |
Journal Title: | Nature Communications |
Publisher: | Nature Publishing Group UK |
Publisher Place: | [London] |
Volume: | 12 |
Original Publication: | 10.1038/s41467-021-25294-z |
Page Start: | 1 |
Page End: | 12 |
Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
File | Description | Size | Format | |
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s41467-021-25294-z.pdf | 7.01 MB | Adobe PDF | ![]() View/Open |