Please use this identifier to cite or link to this item:
http://dx.doi.org/10.25673/113669
Title: | The role of microRNAs in gene expression and signaling response of tumor cells to an acidic environment |
Author(s): | Riemann, Anne![]() Rauschner, Mandy Reime, Sarah Thews, Oliver |
Issue Date: | 2023 |
Type: | Article |
Language: | English |
Abstract: | Many tumors are characterized by marked extracellular acidosis due to increased glycolytic metabolism, which affects gene expression and thereby tumor biological behavior. At the same time, acidosis leads to altered expression of several microRNAs (Mir7, Mir183, Mir203, Mir215). The aim of this study was to analyze whether the acidosis-induced changes in cytokines and tumor-related genes are mediated via pH-sensitive microRNAs. Therefore, the expression of Il6, Nos2, Ccl2, Spp1, Tnf, Acat2, Aox1, Crem, Gls2, Per3, Pink1, Txnip, and Ypel3 was examined in acidosis upon simultaneous transfection with microRNA mimics or antagomirs in two tumor lines in vitro and in vivo. In addition, it was investigated whether microRNA expression in acidosis is affected via known pH-sensitive signaling pathways (MAPK, PKC, PI3K), via ROS, or via altered intracellular Ca2+ concentration. pH-dependent microRNAs were shown to play only a minor role in modulating gene expression. Individual genes (e.g., Ccl2, Txnip, Ypel3) appear to be affected by Mir183, Mir203, or Mir215 in acidosis, but these effects are cell line-specific. When examining whether acid-dependent signaling affects microRNA expression, it was found that Mir203 was modulated by MAPK and ROS, Mir7 was affected by PKC, and Mir215 was dependent on the intracellular Ca2+ concentration. Mir183 could be increased by ROS scavenging. These correlations could possibly result in new therapeutic approaches for acidotic tumors. |
URI: | https://opendata.uni-halle.de//handle/1981185920/115624 http://dx.doi.org/10.25673/113669 |
Open Access: | ![]() |
License: | ![]() |
Journal Title: | International journal of molecular sciences |
Publisher: | Molecular Diversity Preservation International |
Publisher Place: | Basel |
Volume: | 24 |
Issue: | 23 |
Original Publication: | 10.3390/ijms242316919 |
Page Start: | 1 |
Page End: | 21 |
Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
File | Description | Size | Format | |
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ijms-24-16919.pdf | 6.68 MB | Adobe PDF | ![]() View/Open |