Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/116527
Title: Receptor-like cytoplasmic kinases of different subfamilies differentially regulate SOBIR1/BAK1-mediated immune responses in Nicotiana benthamiana
Author(s): Huang, Wen R. H.
Braam, Ciska
Kretschmer, Carola
Landeo Villanueva, Sergio
Liu, HuanLook up in the Integrated Authority File of the German National Library
Ferik, Filiz
Burgh, Aranka M.
Wu, Jinbin
Zhang, LishaLook up in the Integrated Authority File of the German National Library
Nürnberger, ThorstenLook up in the Integrated Authority File of the German National Library
Wang, YuluanLook up in the Integrated Authority File of the German National Library
Seidl, Michael F.
Evangelisti, Edouard
Stuttmann, JohannesLook up in the Integrated Authority File of the German National Library
Joosten, Matthieu H. A. J.
Issue Date: 2024
Type: Article
Language: English
Abstract: Cell-surface receptors form the front line of plant immunity. The leucine-rich repeat (LRR)-receptor-like kinases SOBIR1 and BAK1 are required for the functionality of the tomato LRR-receptor-like protein Cf-4, which detects the secreted effector Avr4 of the pathogenic fungus Fulvia fulva. Here, we show that the kinase domains of SOBIR1 and BAK1 directly phosphorylate each other and that residues Thr522 and Tyr469 of the kinase domain of Nicotiana benthamiana SOBIR1 are required for its kinase activity and for interacting with signalling partners, respectively. By knocking out multiple genes belonging to different receptor-like cytoplasmic kinase (RLCK)-VII subfamilies in N. benthamiana:Cf-4, we show that members of RLCK-VII-6, −7, and −8 differentially regulate the Avr4/Cf-4-triggered biphasic burst of reactive oxygen species. In addition, members of RLCK-VII-7 play an essential role in resistance against the oomycete pathogen Phytophthora palmivora. Our study provides molecular evidence for the specific roles of RLCKs downstream of SOBIR1/BAK1-containing immune complexes.
URI: https://opendata.uni-halle.de//handle/1981185920/118482
http://dx.doi.org/10.25673/116527
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Journal Title: Nature Communications
Publisher: Springer Nature
Publisher Place: [London]
Volume: 15
Original Publication: 10.1038/s41467-024-48313-1
Page Start: 1
Page End: 17
Appears in Collections:Open Access Publikationen der MLU

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