Antimycobacterial characterisation of Nα-aroyl-N-aryl phenylalanine amides with focus on Mycobacterium abscessus

Abstract

The work carried out as part of the dissertation included the establishment of different methods for testing substances on relevant non-tuberculous mycobacteria, such as M. abscessus. In particular, the possibility of using different methods (synergy testing, macrophage-infection-assay, MBC determination) ensured a differentiated characterisation of the substances. This contributed to the optimisation and further development of the potential drug candidate MMV688845, an RNA polymerase inhibitor from the Nα-aroyl-N-aryl-phenylalaninamide substance class. As part of the work, an assay based on fluorescence microscopic evaluation was also developed to simplify the determination of minimal bactericidal concentrations.