Please use this identifier to cite or link to this item:
http://dx.doi.org/10.25673/60207
Title: | A rapid and reliable liquid chromatography/mass spectrometry method for SARS-CoV-2 analysis from gargle solutions and saliva |
Author(s): | Kipping, Marc Tänzler, Dirk Sinz, Andrea |
Issue Date: | 2021 |
Type: | Article |
Language: | English |
Abstract: | We describe a rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the direct detection and quantitation of SARS-CoV-2 nucleoprotein in gargle solutions and saliva. The method is based on a multiple-reaction monitoring (MRM) mass spectrometry approach with a total cycle time of 5 min per analysis and allows the detection and accurate quantitation of SARS-CoV-2 nucleoprotein as low as 500 amol/μL. We improved the sample preparation protocol of our recent piloting SARS-CoV-2 LC-MS study regarding sensitivity, reproducibility, and compatibility with a complementary reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis of the same sample. The aim of this work is to promote diagnostic tools that allow identifying and monitoring SARS-CoV-2 infections by LC-MS/MS methods in a routine clinical environment. |
URI: | https://opendata.uni-halle.de//handle/1981185920/62158 http://dx.doi.org/10.25673/60207 |
Open Access: | ![]() |
License: | ![]() |
Sponsor/Funder: | Publikationsfonds MLU |
Journal Title: | Analytical and Bioanalytical Chemistry |
Publisher: | Springer |
Publisher Place: | Berlin |
Volume: | 413 |
Original Publication: | 10.1007/s00216-021-03614-y |
Page Start: | 6503 |
Page End: | 6511 |
Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
File | Description | Size | Format | |
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Kipping2021_Article_ARapidAndReliableLiquidChromat.pdf | 802.48 kB | Adobe PDF | ![]() View/Open |