https://opendata.uni-halle.de//handle/1981185920/6693 2026-03-16T04:03:19Z https://opendata.uni-halle.de//handle/1981185920/124430 Title: Advancing the potential of the metastatistical extreme value framework for extreme flood estimation in German catchments Author(s): Mushtaq, Sumra Abstract: River floods are among the most destructive natural disasters, with their risk projected to increase due to socioeconomic and climate changes, posing a growing global threat. Heavy-tailed behavior in flood distributions serves as a key indicator of extreme flood likelihood, emphasizing the need for accurate identification and prediction of such patterns. This dissertation advances the understanding of heavy-tailed flood distributions while introducing the Metastatistical Extreme Value (MEV) framework for predicting and managing the extreme flood events in practical applications. A statistical approach that accounts for different runoff-generation processes is applied and tested using daily streamflow time series from 182 streamflow gauges in Germany. The results provide new insights into heavy-tailed flood behavior and offer a robust method for predicting extreme floods. This method is less sensitive to limited data, and is applicable across diverse hydroclimatic conditions. 2025-01-01T00:00:00Z https://opendata.uni-halle.de//handle/1981185920/124425 Title: Specific bromination of fluorinated oligophenylenes, towards cyclo-para- and cyclo-meta-phenylenes Author(s): El Alaoui, Nour-Eddine Abstract: This study investigates the synthesis of two distinct classes of macrocycles: non-fluorinated cyclo-para-phenylenes (CPPs) and fluorinated cyclo-meta-phenylenes (CMPSs). On-Surface Synthesis (CPPs): We synthesized quasi-planar, π-extended CPPs on Au(111). Characterization via STM/STS and DFT. Solution-Phase Synthesis (CMPs): We developed a modular route using selective ironmediated bromination to create fluorinated oligophenylene scaffolds. These precursors undergo nickel-mediated Yamamoto coupling to form fluorinated CMPsThis dualmethodology framework explores how curvature, fluorination, and electronic confinement interact within strained benzenoid architectures. 2026-01-01T00:00:00Z https://opendata.uni-halle.de//handle/1981185920/124424 Title: Dissecting oncogenic signaling networks through combinatorial and single-cell CRISPR screens Author(s): El Kassem, Ghanem Abstract: Understanding how genes act together to control cellular behavior is central to elucidating disease mechanisms. This thesis presents two CRISPR-based platforms to study genetic interactions and transcriptional regulation in human cells. First, we evaluate the RNA-targeting CRISPR effector Cas13d for quantitative genetic interaction mapping. Cas13d enables reversible transcript-level gene silencing and supports efficient dual-gene perturbations. Using a dual-promoter gRNA expression strategy, we mapped interactions among six genes modulating response of the CML cell line K562 to imatinib. Second, we use single-cell CRISPR sequencing to reconstruct the transcriptional network downstream of RAF-MAPK signaling. Perturbation of 22 transcription factors in an inducible RAF1 HEK293 model reveals a feedback loop between EGR1 and TCF7, linking MAPK and Wnt signaling. Together, these approaches enable scalable, high-resolution analysis of genetic dependencies relevant to cancer therapy. 2025-01-01T00:00:00Z https://opendata.uni-halle.de//handle/1981185920/124423 Title: Towards the role of TatA and TatB in the activity and stability of thylakoidal Tat translocase Author(s): Zhou, Ming Abstract: Protein sorting and transport are fundamental processes in eukaryotic cells. In chloroplasts, twin-arginine translocation (Tat) translocase mediates the transport of folded proteins across thylakoid membranes, which consists of TatA, TatB and TatC subunits. Here, in thylakoidal reconstitution assays were used to investigate the roles of individual Tat subunits. Only one of three in vitro translation systems supported functional TatB reconstitution, leading to the use of purified TatA and TatB for further quantitative studies. Unexpectedly, high concentrations of either protein reduced intrinsic Tat transport. BN-PAGE revealed that external TatA destabilizes TatBC complexes, although TatBC signal intensities did not correlate linearly with transport activity. Moreover, TatB could substitute for TatA, whereas TatA could not replace TatB. Finally, the proton motive force was shown to influence TatBC stability, highlighting the dynamic regulation of the chloroplast Tat translocase. 2025-01-01T00:00:00Z