Study of the Relationship between Myeloperoxidase Enzyme, Ghrelin Hormone, and Relaxin Hormone Levels in Blood Serum During Late-Stage Pregnancy

Abstract

To study the effect of levels of amyloperoxidase, ghrelin, and relaxin on the late stages of pregnancy in pregnant women, and to study the correlation between the study variables. To identify potential factors that may lead to oxidative stress in pregnant women during the late stages of pregnancy. Amyloperoxidase is classified as an oxidoreductase enzyme, belongs to the peroxidase family, and contains a heme group. It is found primarily in neutrophils and lymphocytes, as well as in monocytes and macrophages. Approximately 30% of cellular amyloperoxidase is secreted as an active enzyme into the extracellular fluid, along with several antimicrobial factors at the site of inflammation, through the lysis of neutrophils. The study included collecting 90 blood samples, from patients and healthy individuals, during the period from 14/1/2024 to14 /2/2024 from pregnant women in the final stages of pregnancy. The samples were collected from Al-Yarmouk Teaching Hospital, Department of Obstetrics and Gynecology, Baghdad. The samples included 60 patient samples and 30 healthy samples representing the control group. The results were analyzed using an ELISA device, which went through several steps, including preparing the ready-made measurement kit containing antigens for the variables under study. Then, the blood serum containing the antibodies was added, where the reaction process was carried out. The serum was then washed several times, and the results were placed in the device for the purpose of reading the absorbance at a wavelength of 450 nm. Research results: The research results showed a significant increase in the level of the enzyme amyloperoxidase and a decrease in the levels of both relaxin and ghrelin hormones. The current results indicate that there is no correlation between the enzyme myeloperoxidase and both relaxin and ghrelin.