Please use this identifier to cite or link to this item:
Title: Identification and quantification of cannabinol as a biomarker for local hemp retting in an ancient sedimentary record by HPTLC-ESI-MS
Author(s): Schmidt, Theresa
Kramell, Annemarie Elisabeth
Oehler, Florian
Kluge, RalphLook up in the Integrated Authority File of the German National Library
Demske, Dieter
Tarasov, Pavel E.Look up in the Integrated Authority File of the German National Library
Csuk, RenéLook up in the Integrated Authority File of the German National Library
Issue Date: 2020
Type: Article
Language: English
Abstract: Cannabis products have been used in various fields of everyday life for many centuries, and applications in folk medicine and textile production have been well-known for many centuries. For traditional textile production, hemp fibers were extracted from the stems by water retting in stagnant or slow-moving waters. During this procedure, parts of the plant material‚ among them phytocannabinoids‚ are released into the water. Cannabinol (CBN) is an important degradation product of the predominant phytocannabinoids found in Cannabis species. Thus, it is an excellent indicator for present as well as ancient hemp water retting. In this study, we developed and validated a simple and fast method for the determination of CBN in sediment samples using high-performance thin-layer chromatography (HPTLC) combined with electrospray ionization mass spectrometry (ESI-MS), thereby testing different extraction and cleanup procedures‚ as well as various sorbents and solvents for planar chromatography. This method shows a satisfactory overall analytical performance with an average recovery rate of 73%. Our protocol enabled qualitative and quantitative analyses of CBN in samples of a bottom sediment core‚ having been obtained from a small lake in Northern India, where intense local retting of hemp was suggested in the past. The analyses showed a maximum CBN content in pollen zone 4 covering a depth range of 262–209 cm, dating from approximately 480 BCE to 1050 CE. These findings correlate with existing records of Cannabis-type pollen. Thus, the method we propose is a helpful tool to track ancient hemp retting activities.
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Sponsor/Funder: Publikationsfond MLU
Journal Title: Analytical and bioanalytical chemistry
Publisher: Springer
Publisher Place: Berlin
Volume: 412
Issue: 11
Original Publication: 10.1007/s00216-020-02492-0
Page Start: 2633
Page End: 2644
Appears in Collections:Open Access Publikationen der MLU

Files in This Item:
File Description SizeFormat 
Schmidt2020_Article_IdentificationAndQuantificatio.pdf1.25 MBAdobe PDFThumbnail