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http://dx.doi.org/10.25673/60207| Title: | A rapid and reliable liquid chromatography/mass spectrometry method for SARS-CoV-2 analysis from gargle solutions and saliva |
| Author(s): | Kipping, Marc Tänzler, Dirk Sinz, Andrea |
| Issue Date: | 2021 |
| Type: | Article |
| Language: | English |
| Abstract: | We describe a rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the direct detection and quantitation of SARS-CoV-2 nucleoprotein in gargle solutions and saliva. The method is based on a multiple-reaction monitoring (MRM) mass spectrometry approach with a total cycle time of 5 min per analysis and allows the detection and accurate quantitation of SARS-CoV-2 nucleoprotein as low as 500 amol/μL. We improved the sample preparation protocol of our recent piloting SARS-CoV-2 LC-MS study regarding sensitivity, reproducibility, and compatibility with a complementary reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis of the same sample. The aim of this work is to promote diagnostic tools that allow identifying and monitoring SARS-CoV-2 infections by LC-MS/MS methods in a routine clinical environment. |
| URI: | https://opendata.uni-halle.de//handle/1981185920/62158 http://dx.doi.org/10.25673/60207 |
| Open Access: |  Open access publication |
| License: |  (CC BY 4.0) Creative Commons Attribution 4.0 |
| Sponsor/Funder: | Publikationsfonds MLU |
| Journal Title: | Analytical and Bioanalytical Chemistry |
| Publisher: | Springer |
| Publisher Place: | Berlin |
| Volume: | 413 |
| Original Publication: | 10.1007/s00216-021-03614-y |
| Page Start: | 6503 |
| Page End: | 6511 |
| Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Kipping2021_Article_ARapidAndReliableLiquidChromat.pdf | 802.48 kB | Adobe PDF |  View/Open |