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Title: A combined oro-nasopharyngeal swab is more sensitive than mouthwash in detecting SARS-CoV-2 by a high-throughput PCR assay
Author(s): Michel, Wiebke
Färber, JacquelineLook up in the Integrated Authority File of the German National Library
Dilas, Milica
Heuft, Hans-GertLook up in the Integrated Authority File of the German National Library
Tammer, InaLook up in the Integrated Authority File of the German National Library
Baar, Jannik
Kaasch, AchimLook up in the Integrated Authority File of the German National Library
Issue Date: 2021
Type: Article
Language: English
URN: urn:nbn:de:gbv:ma9:1-1981185920-1031026
Subjects: Diagnostic accuracy
Oronasopharyngeal swab
Abstract: Objectives The optimal diagnostic specimen to detect SARS-CoV-2 by PCR in the upper respiratory tract is unclear. Mouthwash fluid has been reported as an alternative to nasopharyngeal and oropharyngeal swabs. We compared mouthwash fluid with a combined oro-nasopharyngeal swab regarding test performance. Methods In a large refugee facility, we retested individuals with a previous positive test for SARS-CoV-2 and their quarantined close contacts. All individuals were asymptomatic at the time of testing. First, a mouthwash (gargling for at least 5 s) with sterile water was performed. Then, with a single flocked swab the back of the throat and subsequently the nasopharynx were sampled. Samples were inactivated and analysed on a Roche cobas 6800 ® system with the Roche SARS-CoV-2 test. Results Of 76 individuals, 39 (51%) tested positive for SARS-CoV-2 by oro-nasopharyngeal swab. Mouthwash detected 13 of 76 (17%) infections, but did not detect any additional infection. Samples that were positive in both tests, had lower cycle threshold (Ct)-values for oro-nasopharyngeal samples, indicating a higher virus concentration, compared to samples only positive in oro-nasopharyngeal swabs. Conclusion Mouthwash is not as sensitive as combined oro-nasopharyngeal swab in detecting upper respiratory tract infection.
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Sponsor/Funder: Projekt DEAL 2021
Journal Title: Infection
Publisher: Urban & Vogel
Publisher Place: München
Volume: 49
Issue: 3
Original Publication: 10.1007/s15010-021-01600-1
Page Start: 527
Page End: 531
Appears in Collections:Medizinische Fakultät (OA)

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