Effect of Green Selenium Nanosynthesis on Staphylococcus Aureus

Abstract

Selenium nanoparticles (SeNPs) represent a promising natural compound for modern medical treatments, offering a potent new weapon against pathogenic bacteria. This study aimed to synthesize SeNPs using sodium selenite (Na₂SeO₃) as a precursor and dried Camellia sinensis (green tea) leaf extract as a reducing and stabilizing agent through an eco-friendly biosynthesis approach. The synthesis was performed via a simple, cost-effective, and environmentally friendly method. The resulting nanoparticles were characterized using UV-visible spectroscopy, field-emission scanning electron microscopy (FE-SEM), and energy-dispersive X-ray spectroscopy (EDX). FE-SEM analysis confirmed the formation of spherical SeNPs with varied shapes and an average particle size of 20.5 nm, alongside a narrow size distribution. While the antibacterial evaluation against Staphylococcus aureus showed no significant inhibitory activity against planktonic cells, the SeNPs exhibited a strong antibiofilm effect, inhibiting biofilm formation by 54.7%. These findings highlight the potential of biosynthesized SeNPs as a specific and effective anti-biofilm agent for targeting bacterial biofilms and combating antibiotic resistance.