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Titel: Fast, inexpensive, and reliable HPLC method to determine monomer fractions in poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
Autor(en): Duvigneau, Stefanie
Kettner, AlexanderIn der Gemeinsamen Normdatei der DNB nachschlagen
Carius, LisaIn der Gemeinsamen Normdatei der DNB nachschlagen
Griehl, CarolaIn der Gemeinsamen Normdatei der DNB nachschlagen
Findeisen, RolfIn der Gemeinsamen Normdatei der DNB nachschlagen
Kienle, AchimIn der Gemeinsamen Normdatei der DNB nachschlagen
Erscheinungsdatum: 2021
Art: Artikel
Sprache: Englisch
URN: urn:nbn:de:gbv:ma9:1-1981185920-1031471
Schlagwörter: Bioplastic
Polyhydroxyalkanoate
Quantitative measurement
High-pressure liquid chromatography
UV/Vis spectrometry
Zusammenfassung: The determination of the monomer fractions in polyhydroxyalkanoates is of great importance for research on microbialproduced plastic material. The development of new process designs, the validation of mathematical models, and intelligent control strategies for production depend enormously on the correctness of the analyzed monomer fractions. Most of the available detection methods focus on the determination of the monomer fractions of the homopolymer poly(3-hydroxybutyrate). Only a few can analyze the monomer content in copolymers such as poly(3-hydroxybutyrate-co-3-hydroxyvalerate), which usually require expensive measuring devices, a high preparation time or the use of environmentally harmful halogenated solvents such as chloroform or dichloromethane. This work presents a fast, simple, and inexpensive method for the analysis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with high-performance liquid chromatography. Samples from a bioreactor experiment for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with Cupriavidus necator H16 were examined regarding their monomer content using the new method and gas chromatography analysis, one of the most frequently used methods in literature. The results from our new method were validated using gas chromatography measurements and show excellent agreement.
URI: https://opendata.uni-halle.de//handle/1981185920/103147
http://dx.doi.org/10.25673/101191
Open-Access: Open-Access-Publikation
Nutzungslizenz: (CC BY 4.0) Creative Commons Namensnennung 4.0 International(CC BY 4.0) Creative Commons Namensnennung 4.0 International
Sponsor/Geldgeber: Projekt DEAL 2021
Journal Titel: Applied microbiology and biotechnology
Verlag: Springer
Verlagsort: Berlin
Band: 105
Heft: 5
Originalveröffentlichung: 10.1007/s00253-021-11265-3
Seitenanfang: 4743
Seitenende: 4749
Enthalten in den Sammlungen:Fakultät für Elektrotechnik und Informationstechnik (OA)

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