Determination of Tetracycline Hydrochloride by Using Dapsone as a Reagent in Developed Spectrophotometric Analysis

Abstract

Inexpensive spectrophotometric method has been developed to estimate its pure concentration. This method is based on the azo coupling reaction using Diaz onium dapsone, which is formed in a solution containing hydrochloric acid and sodium nitrate. The resulting salt reacts with tetracycline hydrochloride to form a Tetracycline hydrochloride is considered an antibiotic, and a sensitive, easy, fast, and orange azo dye at a wavelength of 380 nanometres. After optimizing the reaction conditions, a recovery rate of 100% wasachieved, with a Beer-Lambert limit between (2 - 26) μg /mL, and a Sandal sensitivity of 0.0238 μg.cm². The method also demonstrated high sensitivity without any interference from the excipients present in the formulation, with a sensitivity value of 20160 L·mol⁻¹·cm⁻¹ and a correlation coefficient of 0.9992. The limit of detection (LOD) was found to be 0.094 and the limit of quantification (LOQ) was found to be 0.28. The reaction was stable for a sufficient period, making the method suitable for estimating tetracycline in its pure form and in harmaceutical preparations, such as capsules, without any interference.