Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/36431
Title: The TFF peptides xP1 and xP4 appear in distinctive forms in the Xenopus laevis gastric mucosa : indications for different protective functions
Author(s): Stürmer, René
Reising, Jana
Hoffmann, Werner
Issue Date: 2019
Type: Article
Language: English
URN: urn:nbn:de:gbv:ma9:1-1981185920-366637
Subjects: Trefoil factor
TFF1
TFF2
Gastric protection
Oxidative stress
ROS
Mucin
Lecithin
Thiol
Abstract: The gastric secretory trefoil factor family (TFF) peptides xP1 and xP4 are the Xenopus laevis orthologs of mammalian TFF1 and TFF2, respectively. The aim of this study was to analyze the molecular forms of xP1 and xP4 in the X. laevis gastric mucosa by FPLC. xP1 mainly occurred in a monomeric low-molecular-mass form and only a minor subset is associated with the mucus fraction. The occurrence of monomeric xP1 is unexpected because of its odd number of cysteine residues. Probably a conserved acidic residue flanking Cys55 allows monomeric secretion. Furthermore, Cys55 is probably post-translationally modified. For the first time, we hypothesize that the free thiol of monomeric xP1-and probably also its mammalian ortholog TFF1-could have a protective scavenger function, e.g., for reactive oxygen/nitrogen species. In contrast, xP4 mainly occurs in a high-molecular-mass form and is non-covalently bound to a mucin similarly as TFF2. In vitro binding studies with radioactively labeled porcine TFF2 even showed binding to X. laevis gastric mucin. Thus, xP4 is expected to bind as a lectin to an evolutionary conserved sugar epitope of the X. laevis ortholog of mucin MUC6 creating a tight mucus barrier. Taken together, xP1 and xP4 appear to have different gastric protective functions.
URI: https://opendata.uni-halle.de//handle/1981185920/36663
http://dx.doi.org/10.25673/36431
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Sponsor/Funder: DFG-Publikationsfonds 2020
Journal Title: International journal of molecular sciences
Publisher: Molecular Diversity Preservation International
Publisher Place: Basel
Volume: 20
Issue: 23
Original Publication: 10.3390/ijms20236052
Page Start: 1
Page End: 9
Appears in Collections:Medizinische Fakultät (OA)

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