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http://dx.doi.org/10.25673/85325
Titel: | Plasma membrane Ca2+ ATPase 1 (PMCA1) but not PMCA4 is critical for B-cell development and Ca2+ homeostasis in mice |
Autor(en): | Korthals, Mark Tech, Laura Langnaese, Kristina Gottfried, Anna ![]() Hradsky, Johannes ![]() Thomas, Ulrich Zenclussen, Ana Claudia ![]() Brunner-Weinzierl, Monika ![]() Tedford, Kerry ![]() Fischer, Klaus-Dieter ![]() |
Erscheinungsdatum: | 2021 |
Art: | Artikel |
Sprache: | Englisch |
URN: | urn:nbn:de:gbv:ma9:1-1981185920-872779 |
Schlagwörter: | B cells Ca2+ PMCA1 PMCA4 Development |
Zusammenfassung: | The amplitude and duration of Ca2+ signaling is crucial for B-cell development and self-tolerance; however, the mechanisms for terminating Ca2+ signals in B cells have not been determined. In lymphocytes, plasma membrane Ca2+ ATPase (PMCA) isoforms 1 and 4 (PMCA1 and PMCA4, aka ATP2B1 and ATP2B4) are the main candidates for expelling Ca2+ from the cell through the plasma membrane. We report here that Pmca4 (Atp2b4) KO mice had normal B-cell development, while mice with a conditional KO of Pmca1 (Atp2b1) had greatly reduced numbers of B cells, particularly splenic follicular B cells, marginal zone B cells, and peritoneal B-1a cells. Mouse and naïve human B cells showed only PMCA1 expression and no PMCA4 by western blot, in contrast to T cells, which did express PMCA4. Calcium handling was normal in Pmca4−/− B cells, but Pmca1 KO B cells had elevated basal levels of Ca2+, elevated levels in ER stores, and reduced Ca2+ clearance. These findings show that the PMCA1 isoform alone is required to ensure normal B-cell Ca2+ signaling and development, which may have implications for therapeutic targeting of PMCAs and Ca2+ in B cells. |
URI: | https://opendata.uni-halle.de//handle/1981185920/87277 http://dx.doi.org/10.25673/85325 |
Open-Access: | ![]() |
Nutzungslizenz: | ![]() |
Sponsor/Geldgeber: | Projekt DEAL 2020 |
Journal Titel: | European journal of immunology |
Verlag: | Wiley-VCH |
Verlagsort: | Weinheim |
Band: | 51 |
Heft: | 3 |
Originalveröffentlichung: | 10.1002/eji.202048654 |
Seitenanfang: | 594 |
Seitenende: | 602 |
Enthalten in den Sammlungen: | Medizinische Fakultät (OA) |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Korthals et al._Plasma membrane_2021.pdf | Zweitveröffentlichung | 1.74 MB | Adobe PDF | ![]() Öffnen/Anzeigen |