Please use this identifier to cite or link to this item:
http://dx.doi.org/10.25673/101628
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DC Field | Value | Language |
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dc.contributor.author | Behringer, Akhil | - |
dc.contributor.author | Stoimenovski, Darko | - |
dc.contributor.author | Porsch, Martin | - |
dc.contributor.author | Hoffmann, Katrin | - |
dc.contributor.author | Behre, Gerhard | - |
dc.contributor.author | Große, Ivo | - |
dc.contributor.author | Kalinski, Thomas | - |
dc.contributor.author | Haybäck, Johannes | - |
dc.contributor.author | Naß, Norbert | - |
dc.date.accessioned | 2023-03-31T06:47:16Z | - |
dc.date.available | 2023-03-31T06:47:16Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | https://opendata.uni-halle.de//handle/1981185920/103575 | - |
dc.identifier.uri | http://dx.doi.org/10.25673/101628 | - |
dc.description.abstract | Background: Tamoxifen-adapted MCF-7-Tam cells represent an in-vitro model for acquired tamoxifen resistance, which is still a problem in clinics. We here investigated the correlation of microRNA-, mRNA- and eukaryotic initiation factors (eIFs) expression in this model. Methods: MicroRNA- and gene expression were analyzed by nCounter and qRT-PCR technology; eIFs by Western blotting. Protein translation mode was determined using a reporter gene assay. Cells were transfected with a miR-1972-mimic. Results: miR-181b-5p,-3p and miR-455-5p were up-, miR-375, and miR-1972 down-regulated and are significant in survival analysis. About 5% of the predicted target genes were significantly altered. Pathway enrichment analysis suggested a contribution of the FoxO1 pathway. The ratio of polio-IRES driven to cap-dependent protein translation shifted towards cap-dependent initiation. Protein expression of eIF2A, -4G, -4H and -6 decreased, whereas eIF3H was higher in MCF-7-Tam. Significant correlations between tamoxifen-regulated miRNAs and eIFs were found in representative breast cancer cell lines. Transfection with a miR-1972-mimic reverses tamoxifen-induced expression for a subset of genes and increased proliferation in MCF-7, but reduced proliferation in MCF-7-Tam, especially in the presence of 4OH-tamoxifen. Migration was inhibited in MCF-7-Tam cells. Translation mode remained unaffected. Conclusions: miR-1972 contributes to the orchestration of gene-expression and physiological consequences of tamoxifen adaption. | eng |
dc.language.iso | eng | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject.ddc | 611 | - |
dc.title | Relationship of micro-RNA, mRNA and eIF expression in tamoxifen-adapted MCF-7 breast cancer cells : impact of miR-1972 on gene expression, proliferation and migration | eng |
dc.type | Article | - |
local.versionType | publishedVersion | - |
local.bibliographicCitation.journaltitle | Biomolecules | - |
local.bibliographicCitation.volume | 12 | - |
local.bibliographicCitation.issue | 7 | - |
local.bibliographicCitation.pagestart | 1 | - |
local.bibliographicCitation.pageend | 20 | - |
local.bibliographicCitation.publishername | MDPI | - |
local.bibliographicCitation.publisherplace | Basel | - |
local.bibliographicCitation.doi | 10.3390/biom12070916 | - |
local.openaccess | true | - |
dc.identifier.ppn | 1812851111 | - |
local.bibliographicCitation.year | 2022 | - |
cbs.sru.importDate | 2023-03-31T06:46:45Z | - |
local.bibliographicCitation | Enthalten in Biomolecules - Basel : MDPI, 2011 | - |
local.accessrights.dnb | free | - |
Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
File | Description | Size | Format | |
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biomolecules-12-00916.pdf | 3.91 MB | Adobe PDF | View/Open |