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Title: Germline immortality relies on TRIM32-mediated turnover of a maternal mRNA activator in C. elegans
Author(s): Oyewale, Tosin D.
Eckmann, Christian R.Look up in the Integrated Authority File of the German National Library
Issue Date: 2022
Type: Article
Language: English
Abstract: How the germ line achieves a clean transition from maternal to zygotic gene expression control is a fundamental problem in sexually reproducing organisms. Whereas several mechanisms terminate the maternal program in the soma, this combined molecular reset and handover are poorly understood for primordial germ cells (PGCs). Here, we show that GRIF-1, a TRIM32-related and presumed E3 ubiquitin ligase in Caenorhabditis elegans, eliminates the maternal cytoplasmic poly(A) polymerase (cytoPAP) complex by targeting the germline-specific intrinsically disordered region of its enzymatic subunit, GLD-2, for proteasome-mediated degradation. Interference with cytoPAP turnover in PGCs causes frequent transgenerational sterility and, eventually, germline mortality. Hence, positively acting maternal RNA regulators are cleared via the proteasome system to avoid likely interference between maternal and zygotic gene expression programs to maintain transgenerational fertility and acquire germline immortality. This strategy is likely used in all animals that preform their immortal germ line via maternally inherited germplasm determinants.
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Journal Title: Science advances
Publisher: Assoc.
Publisher Place: Washington, DC [u.a.]
Volume: 8
Issue: 41
Original Publication: 10.1126/sciadv.abn0897
Page Start: 1
Page End: 15
Appears in Collections:Open Access Publikationen der MLU

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