Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/101874
Title: Intrinsically cross-linked ECM-like multilayers for BMP-2 delivery promote osteogenic differentiation of cells
Author(s): Anouz, Reema
Selekere, Tamaradobra
Hautmann, Adrian
Husteden, Catharina
Menzel, Matthias
Woelk, Christian
Schmelzer, Christian E. H.
Groth, ThomasLook up in the Integrated Authority File of the German National Library
Issue Date: 2023
Type: Article
Language: English
Abstract: Surface coatings prepared by layer-by-layer technique permit loading of growth factors (GFs) and their spatially controlled release. Here, native chondroitin sulfate (nCS), oxidized CS (oCS100), or mixture of both (oCS50) are combined with collagen I (Col I) to fabricate polyelectrolyte multilayers (PEMs) that exhibit structural, mechanical, and biochemical cues like the natural extracellular-matrix. The use of oCS enables intrinsic cross-linking of PEM that offers higher stability, stiffness, and better control of bone morphogenetic protein-2 (BMP-2) release compared to nCS. oCS100 PEMs have enhanced stiffness, promote Col I fibrillization, and present BMP-2 in a matrix-bound manner. oCS50 PEMs show intermediate effects on osteogenesis, soft surface, high water content but also moderately slow BMP-2 release profile. C2C12 myoblasts used for osteogenesis studies show that oCS PEMs are more stable and superior to nCS PEMs in supporting cell adhesion and spreading as well as in presenting BMP-2 to the cells. oCS PEMs are triggering more osteogenesis as proved by the quantitative real-time polymerase chain reaction, immune and histochemical staining. These findings show that intrinsic cross-linking in oCS/Col I multilayers provides a successful tool to control GFs delivery and subsequent cell differentiation which opens new opportunities in regenerative therapies of bone and other tissues.
URI: https://opendata.uni-halle.de//handle/1981185920/103825
http://dx.doi.org/10.25673/101874
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Journal Title: Advanced materials interfaces
Publisher: Wiley-VCH
Publisher Place: Weinheim
Volume: 10
Issue: 7
Original Publication: 10.1002/admi.202201596
Appears in Collections:Open Access Publikationen der MLU