Please use this identifier to cite or link to this item:
http://dx.doi.org/10.25673/108830
Title: | Chromosome-scale genome assembly for the duckweed Spirodela intermedia, integrating cytogenetic maps, PacBio and Oxford Nanopore libraries |
Author(s): | Hoang, Phuong T. N. Fiebig, Anne Novák, Petr Macas, Jiří Cao, Hieu X. Stepanenko, Anton Chen, Guimin Borisjuk, Nikolai Scholz, Uwe Schubert, Ingo A. |
Issue Date: | 2020 |
Type: | Article |
Language: | English |
Abstract: | Duckweeds are small, free-floating, morphologically highly reduced organisms belonging to the monocot order Alismatales. They display the most rapid growth among flowering plants, vary ~ 14-fold in genome size and comprise five genera. Spirodela is the phylogenetically oldest genus with only two mainly asexually propagating species: S. polyrhiza (2n = 40; 160 Mbp/1C) and S. intermedia (2n = 36; 160 Mbp/1C). This study combined comparative cytogenetics and de novo genome assembly based on PacBio, Illumina and Oxford Nanopore (ON) reads to obtain the first genome reference for S. intermedia and to compare its genomic features with those of the sister species S. polyrhiza. Both species’ genomes revealed little more than 20,000 putative protein-coding genes, very low rDNA copy numbers and a low amount of repetitive sequences, mainly Ty3/gypsy retroelements. The detection of a few new small chromosome rearrangements between both Spirodela species refined the karyotype and the chromosomal sequence assignment for S. intermedia. |
URI: | https://opendata.uni-halle.de//handle/1981185920/110785 http://dx.doi.org/10.25673/108830 |
Open Access: | Open access publication |
License: | (CC BY 4.0) Creative Commons Attribution 4.0 |
Journal Title: | Scientific reports |
Publisher: | Macmillan Publishers Limited, part of Springer Nature |
Publisher Place: | [London] |
Volume: | 10 |
Original Publication: | 10.1038/s41598-020-75728-9 |
Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
File | Description | Size | Format | |
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s41598-020-75728-9.pdf | 2.99 MB | Adobe PDF | View/Open |