Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/108830
Title: Chromosome-scale genome assembly for the duckweed Spirodela intermedia, integrating cytogenetic maps, PacBio and Oxford Nanopore libraries
Author(s): Hoang, Phuong T. N.
Fiebig, AnneLook up in the Integrated Authority File of the German National Library
Novák, Petr
Macas, Jiří
Cao, Hieu X.
Stepanenko, Anton
Chen, Guimin
Borisjuk, Nikolai
Scholz, Uwe
Schubert, Ingo A.Look up in the Integrated Authority File of the German National Library
Issue Date: 2020
Type: Article
Language: English
Abstract: Duckweeds are small, free-floating, morphologically highly reduced organisms belonging to the monocot order Alismatales. They display the most rapid growth among flowering plants, vary ~ 14-fold in genome size and comprise five genera. Spirodela is the phylogenetically oldest genus with only two mainly asexually propagating species: S. polyrhiza (2n = 40; 160 Mbp/1C) and S. intermedia (2n = 36; 160 Mbp/1C). This study combined comparative cytogenetics and de novo genome assembly based on PacBio, Illumina and Oxford Nanopore (ON) reads to obtain the first genome reference for S. intermedia and to compare its genomic features with those of the sister species S. polyrhiza. Both species’ genomes revealed little more than 20,000 putative protein-coding genes, very low rDNA copy numbers and a low amount of repetitive sequences, mainly Ty3/gypsy retroelements. The detection of a few new small chromosome rearrangements between both Spirodela species refined the karyotype and the chromosomal sequence assignment for S. intermedia.
URI: https://opendata.uni-halle.de//handle/1981185920/110785
http://dx.doi.org/10.25673/108830
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Journal Title: Scientific reports
Publisher: Macmillan Publishers Limited, part of Springer Nature
Publisher Place: [London]
Volume: 10
Original Publication: 10.1038/s41598-020-75728-9
Appears in Collections:Open Access Publikationen der MLU

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