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Title: Differential gene expression in cisplatin-resistant and -sensitive testicular germ cell tumor cell lines
Author(s): Roška, Jan
Wachsmannová, Lenka
Hurbanová, Lenka
Šestáková, Zuzana
Müller, ThomasLook up in the Integrated Authority File of the German National Library
Jurkovičová, Dana
Chovanec, Miroslav
Issue Date: 2020
Type: Article
Language: English
Abstract: Testicular germ cell tumors (TGCTs) represent a well curable malignity due to their exceptional response to cisplatin (CDDP). Despite remarkable treatment results, approximately 5% of TGCT patients develop CDDP resistance and die. Exceptional curability makes TGCTs a highly valuable model system for studying the molecular mechanisms of CDDP sensitivity. Our study was aimed at revealing difference in gene expression between the CDDP-resistant and -sensitive TGCT cell lines, and hence at identifying candidate genes that could serve as potential biomarkers of CDDP response. Using gene expression array, we identified 281 genes that are differentially expressed in CDDP-resistant compared to -sensitive TGCT cell lines. The expression of 25 genes with the highest fold change was validated by RT-qPCR. Of them, DNMT3L, GAL, IGFBP2, IGFBP7, L1TD1, NANOG, NTF3, POU5F1, SOX2, WNT6, ZFP42, ID2, PCP4, SLC40A1 and TRIB3, displayed comparable expression change in gene expression array and RT-qPCR, when all CDDP-resistant TGCT cell lines were pairwise combined with all -sensitive ones. Products of the identified genes are pluripotency factors, or are involved in processes, such as cell metabolism, proliferation or migration. We propose that, after clinical validation, these genes could serve as prognostic biomarkers for early detection of CDDP response in TGCT patients.
Open Access: Open access publication
License: (CC BY 3.0) Creative Commons Attribution 3.0 Unported(CC BY 3.0) Creative Commons Attribution 3.0 Unported
Journal Title: OncoTarget
Publisher: Impact Journals LLC
Publisher Place: [S.l.]
Volume: 11
Issue: 51
Original Publication: 10.18632/oncotarget.27844
Page Start: 4735
Page End: 4753
Appears in Collections:Open Access Publikationen der MLU

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