Please use this identifier to cite or link to this item:
Title: Establishment of vestibular schwannoma primary cell cultures obtained from cavitron ultrasonic surgical aspirator tissue material
Author(s): Leisz, SandraLook up in the Integrated Authority File of the German National Library
Klause, Clara Helene
Becker, Anna-Louisa
Scheer, Maximilian
Simmermacher, Sebastian
Strauss, Christian
Scheller, ChristianLook up in the Integrated Authority File of the German National Library
Issue Date: 2023
Type: Article
Language: English
Abstract: Background: Vestibular schwannoma (VS) is a benign tumor arising from the Schwann cells of the eighth cranial nerve. The complexity in treatment is associated with unpredictable progression of this tumor. Some of the VS do not alter for years, while others rapidly increase in size. The mechanisms behind size progression are not well studied. Furthermore, despite several studies, there is no pharmacological treatment available for sporadic VS. Therefore, in vitro models are essential tools to study the cellular and molecular processes of VS. In addition, patient-derived cell cultures are important for substance screening to investigate pharmacological approaches in vitro. New method: This study presents a simple and fast method for culturing VS cells from patient tissue material obtained using a cavitron ultrasonic surgical aspirator (CUSA). In addition, the cells were characterized based on the expression of schwannoma markers, growth properties and screened for fibroblast contamination. Result: We could show that CUSA obtained material is a suitable resource for isolation of VS primary cultures and enables real time analysis on living cells. Comparison with Existing Methods: To date, only a few protocols are available for culturing VS cells from patient tissue material. A disadvantage of these methods is the relatively large amount of tissue needed to obtain the primary cells, which can be difficult, especially in small VS. By obtaining the cells from the CUSA, there is the possibility to establish a primary culture even with limited material. Conclusion: This approach could be particularly useful for testing substances that represent candidates for drug therapy of vestibular schwannoma.
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Journal Title: Journal of neuroscience methods
Publisher: Elsevier Science
Publisher Place: Amsterdam [u.a.]
Volume: 397
Original Publication: 10.1016/j.jneumeth.2023.109955
Appears in Collections:Open Access Publikationen der MLU

Files in This Item:
File Description SizeFormat 
1-s2.0-S0165027023001747-main.pdf1.92 MBAdobe PDFThumbnail