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http://dx.doi.org/10.25673/113661
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DC Element | Wert | Sprache |
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dc.contributor.author | Blümke, Juliane | - |
dc.contributor.author | Bauer, Marcus | - |
dc.contributor.author | Vaxevanis, Christoforos | - |
dc.contributor.author | Wilfer, Andreas | - |
dc.contributor.author | Mandelboim, Ofer | - |
dc.contributor.author | Wickenhauser, Claudia | - |
dc.contributor.author | Seliger, Barbara | - |
dc.contributor.author | Jasinski-Bergner, Simon | - |
dc.date.accessioned | 2024-01-26T07:33:01Z | - |
dc.date.available | 2024-01-26T07:33:01Z | - |
dc.date.issued | 2023 | - |
dc.identifier.uri | https://opendata.uni-halle.de//handle/1981185920/115616 | - |
dc.identifier.uri | http://dx.doi.org/10.25673/113661 | - |
dc.description.abstract | The human Epstein–Barr virus (EBV), as a member of the human γ herpes viruses (HHV), is known to be linked with distinct tumor types. It is a double-stranded DNA virus and its genome encodes among others for 48 different microRNAs (miRs). Current research demonstrated a strong involvement of certain EBV-miRs in molecular immune evasion mechanisms of infected cells by, e.g., the disruption of human leukocyte antigen (HLA) class Ia and NKG2D functions. To determine novel targets of EBV-miRs involved in immune surveillance, ebv-miR-BART7-3p, an EBV-encoded miR with high expression levels during the different lytic and latent EBV life cycle phases, was overexpressed in human HEK293T cells. Using a cDNA microarray-based comparative analysis, 234 (229 downregulated and 5 upregulated) deregulated human transcripts were identified in ebv-miR-BART7-3p transfectants, which were mainly involved in cellular processes and molecular binding. A statistically significant downregulation of the anti-proliferative and tumor-suppressive hsa-miR-34A and the anti-viral interferon lambda (IFNL)3 mRNA was found. The ebv-miR-BART7-3p-mediated downregulation of IFNL3 expression was due to a direct interaction with the IFNL3 3’-untranslated region (UTR) as determined by luciferase reporter gene assays including the identification of the accurate ebv-miR-BART7-3p binding site. The effect of ebv-miR-BART7-3p on the IFNL3 expression was validated both in human cell lines in vitro and in human tissue specimen with known EBV status. These results expand the current knowledge of EBV-encoded miRs and their role in immune evasion, pathogenesis and malignant transformation. | eng |
dc.language.iso | eng | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | - |
dc.subject.ddc | 610 | - |
dc.title | Identification and characterization of the anti-viral interferon lambda 3 as direct target of the Epstein-Barr virus microRNA-BART7-3p | eng |
dc.type | Article | - |
local.versionType | publishedVersion | - |
local.bibliographicCitation.journaltitle | OncoImmunology | - |
local.bibliographicCitation.volume | 12 | - |
local.bibliographicCitation.issue | 1 | - |
local.bibliographicCitation.pagestart | 1 | - |
local.bibliographicCitation.pageend | 11 | - |
local.bibliographicCitation.publishername | Taylor & Franics | - |
local.bibliographicCitation.publisherplace | Abingdon | - |
local.bibliographicCitation.doi | 10.1080/2162402x.2023.2284483 | - |
local.subject.keywords | EBV; EBV target genes; IFNL3; immune escape; microRNA | - |
local.openaccess | true | - |
dc.identifier.ppn | 1879042878 | - |
cbs.publication.displayform | 2023 | - |
local.bibliographicCitation.year | 2023 | - |
cbs.sru.importDate | 2024-01-26T07:32:08Z | - |
local.bibliographicCitation | Enthalten in OncoImmunology - Abingdon : Taylor & Franics, 2012 | - |
local.accessrights.dnb | free | - |
Enthalten in den Sammlungen: | Open Access Publikationen der MLU |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Identification and characterization of the anti-viral interferon lambda 3 as direct target of the Epstein-Barr virus microRNA-BART7-3p.pdf | 3.4 MB | Adobe PDF | ![]() Öffnen/Anzeigen |