Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/117817
Title: Cytokine adsorption during ex vivo blood perfusion improves contractility of donation after circulatory death hearts
Author(s): Saemann, LarsLook up in the Integrated Authority File of the German National Library
Pohl, SabineLook up in the Integrated Authority File of the German National Library
Wachter, KristinaLook up in the Integrated Authority File of the German National Library
Georgevici, Adrian-IustinLook up in the Integrated Authority File of the German National Library
Köhler, ConnyLook up in the Integrated Authority File of the German National Library
Jünger, JenniferLook up in the Integrated Authority File of the German National Library
Hoorn, FabioLook up in the Integrated Authority File of the German National Library
Gharpure, Nitin
Großkopf, Anne
Korkmaz-İçöz, SevilLook up in the Integrated Authority File of the German National Library
Wenzel, FolkerLook up in the Integrated Authority File of the German National Library
Karck, MatthiasLook up in the Integrated Authority File of the German National Library
Simm, AndreasLook up in the Integrated Authority File of the German National Library
Szabó, GáborLook up in the Integrated Authority File of the German National Library
Issue Date: 2024
Type: Article
Language: English
Abstract: BackgroundDonation after circulatory death (DCD) hearts have to withstand ischemia/reperfusion injury that is partially driven by proinflammatory cytokines and decreases ventricular contractility. We hypothesize that cytokine adsorption during normothermic ex vivo blood perfusion of DCD hearts reduces the cytokine levels and improves ventricular contractility. Methods and ResultsPorcine DCD hearts were maintained 4 hours by ex vivo blood perfusion with (DCD‐BPCytoS, all groups: N=8) or without (DCD‐BP) CytoSorb, followed by 2 hours reperfusion with fresh blood, including left ventricular functional analysis using a balloon catheter. In a control and a DCD group, hearts were evaluated after procurement. We determined lactate and cytokines after ex vivo blood perfusion and the myocardial and left anterior descending artery transcriptome using microarrays after reperfusion. In DCD‐BPCytoS, the developed pressure (control: 124±7 mm Hg/s, DCD: 86±4 mm Hg/s, DCD‐BP: 69±11 mm Hg/s, DCD‐BPCytoS: 112±9 mm Hg/s; P<0.05) and maximal slope of pressure increment (control: 2010±39 mm Hg/s, DCD: 1219±164 mm Hg/s, DCD‐BP: 964±163 mm Hg/s, DCD‐BPCytoS: 1794±205 mm Hg/s; P<0.05) were higher compared with DCD‐BP and DCD hearts. However, contractility decreased later during reperfusion without CytoSorb. After 4 hours, troponin, lactate (45±5% versus 69±9%, P<0.05), IL (interleukin)‐1β, ‐1ra, and ‐8 were lower in DCD‐BPCytoS hearts. In the myocardium of DCD‐BPCytoS compared with DCD‐BP hearts, inflammatory mediator receptor activity/binding pathways were enriched, and pathways for collagen‐containing extracellular matrix and contractile fiber were underrepresented. In the left anterior descending artery of DCD‐BPCytoS hearts, serine/threonine/tyrosine kinase activity and wound‐healing pathways were enriched, and mitochondrial protein‐containing complex and respiratome‐associated pathways were underrepresented. ConclusionsCytoSorb during ex vivo blood perfusion enhances the maintenance of DCD hearts and is likely to improve graft function after transplantation.
URI: https://opendata.uni-halle.de//handle/1981185920/119777
http://dx.doi.org/10.25673/117817
Open Access: Open access publication
License: (CC BY-NC-ND 4.0) Creative Commons Attribution NonCommercial NoDerivatives 4.0(CC BY-NC-ND 4.0) Creative Commons Attribution NonCommercial NoDerivatives 4.0
Journal Title: Journal of the American Heart Association
Publisher: Association
Publisher Place: New York, NY
Volume: 13
Original Publication: 10.1161/jaha.124.036872
Page Start: 1
Page End: 17
Appears in Collections:Open Access Publikationen der MLU