Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/118180
Title: WHIRLY1 regulates aliphatic glucosinolate biosynthesis in early seedling development of arabidopsis
Author(s): Nguyen Thuy LinhLook up in the Integrated Authority File of the German National Library
Moutesidi, PinelopiLook up in the Integrated Authority File of the German National Library
Ziegler, JörgLook up in the Integrated Authority File of the German National Library
Glasneck, Anike
Khosravi, SolmazLook up in the Integrated Authority File of the German National Library
Abel, SteffenLook up in the Integrated Authority File of the German National Library
Hensel, GötzLook up in the Integrated Authority File of the German National Library
Krupinska, KarinLook up in the Integrated Authority File of the German National Library
Humbeck, KlausLook up in the Integrated Authority File of the German National Library
Issue Date: 2025
Type: Article
Language: English
Abstract: WHIRLY1 belongs to a family of plant-specific transcription factors capable of binding DNA or RNA in all three plant cell compartments that contain genetic materials. In Arabidopsis thaliana, WHIRLY1 has been studied at the later stages of plant development, including flowering and leaf senescence, as well as in biotic and abiotic stress responses. In this study, WHIRLY1 knockout mutants of A. thaliana were prepared by CRISPR/Cas9-mediated genome editing to investigate the role of WHIRLY1 during early seedling development. The loss-of-function of WHIRLY1 in 5-day-old seedlings did not cause differences in the phenotype and the photosynthetic performance of the emerging cotyledons compared with the wild type. Nevertheless, comparative RNA sequencing analysis revealed that the knockout of WHIRLY1 affected the expression of a small but specific set of genes during this critical phase of development. About 110 genes were found to be significantly deregulated in the knockout mutant, wherein several genes involved in the early steps of aliphatic glucosinolate (GSL) biosynthesis were suppressed compared with wild-type plants. The downregulation of these genes in WHIRLY1 knockout lines led to decreased GSL contents in seedlings and in seeds. Since GSL catabolism mediated by myrosinases was not altered during seed-to-seedling transition, the results suggest that AtWHIRLY1 plays a major role in modulation of aliphatic GSL biosynthesis during early seedling development. In addition, phylogenetic analysis revealed a coincidence between the evolution of methionine-derived aliphatic GSLs and the addition of a new WHIRLY in core families of the plant order Brassicales.
URI: https://opendata.uni-halle.de//handle/1981185920/120139
http://dx.doi.org/10.25673/118180
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Journal Title: The plant journal
Publisher: Wiley-Blackwell
Publisher Place: Oxford [u.a.]
Volume: 121
Issue: 1
Original Publication: 10.1111/tpj.17181
Appears in Collections:Open Access Publikationen der MLU