Please use this identifier to cite or link to this item:
Title: Slow ring flips in aromatic cluster of GB1 studied by aromatic 13C relaxation dispersion methods
Author(s): Dreydoppel, Matthias
Raum, Heiner N.
Weininger, UlrichLook up in the Integrated Authority File of the German National Library
Issue Date: 2020
Type: Article
Language: English
Abstract: Ring flips of phenylalanine and tyrosine are a hallmark of protein dynamics. They report on transient breathing motions of proteins. In addition, flip rates also depend on stabilizing interactions in the ground state, like aromatic stacking or cation–π interaction. So far, experimental studies of ring flips have almost exclusively been performed on aromatic rings without stabilizing interactions. Here we investigate ring flip dynamics of Phe and Tyr in the aromatic cluster in GB1. We found that all four residues of the cluster, Y3, F30, Y45 and F52, display slow ring flips. Interestingly, F52, the central residue of the cluster, which makes aromatic contacts with all three others, is flipping significantly faster, while the other rings are flipping with the same rates within margin of error. Determined activation enthalpies and activation volumes of these processes are in the same range of other reported ring flips of single aromatic rings. There is no correlation of the number of aromatic stacking interactions to the activation enthalpy, and no correlation of the ring’s extent of burying to the activation volume. Because of these findings, we speculate that F52 is undergoing concerted ring flips with each of the other rings.
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Sponsor/Funder: Publikationsfond MLU
Journal Title: Journal of biomolecular NMR
Publisher: Springer Science + Business Media B.V
Publisher Place: Dordrecht [u.a.]
Volume: 74
Issue: 2/3
Original Publication: 10.1007/s10858-020-00303-3
Page Start: 183
Page End: 191
Appears in Collections:Open Access Publikationen der MLU

Files in This Item:
File Description SizeFormat 
Dreydoppel2020_Article_SlowRingFlipsInAromaticCluster.pdf1.28 MBAdobe PDFThumbnail