Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/73845
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dc.contributor.authorDorner, Julia-
dc.contributor.authorKorn, Patricia-
dc.contributor.authorGruhle, Kai-
dc.contributor.authorRamsbeck, Daniel-
dc.contributor.authorGaramus, Vasil M.-
dc.contributor.authorLilie, Hauke-
dc.contributor.authorMeister, Annette-
dc.contributor.authorSchwieger, Christian-
dc.contributor.authorIhling, Christian-
dc.contributor.authorSinz, Andrea-
dc.contributor.authorDrescher, Simon-
dc.date.accessioned2022-03-08T12:21:48Z-
dc.date.available2022-03-08T12:21:48Z-
dc.date.issued2021-
dc.identifier.urihttps://opendata.uni-halle.de//handle/1981185920/75797-
dc.identifier.urihttp://dx.doi.org/10.25673/73845-
dc.description.abstractAlthough incorporation of photo-activatable lipids into membranes potentially opens up novel avenues for investigating interactions with proteins, the question of whether diazirine-modified lipids are suitable for such studies, remains under debate. Focusing on the potential for studying lipid/peptide interactions by cross-linking mass spectrometry (XL-MS), we developed a diazirine-modified lipid (DiazPC), and examined its behaviour in membranes incorporating the model α-helical peptide LAVA20. We observed an unexpected backfolding of the diazirine-containing stearoyl chain of the lipid. This surprising behaviour challenges the potential application of DiazPC for future XL-MS studies of peptide and protein/lipid interactions. The observations made for DiazPC most likely represent a general phenomenon for any type of membrane lipids with a polar moiety incorporated into the alkyl chain. Our finding is therefore of importance for future protein/lipid interaction studies relying on modified lipid probes.eng
dc.description.sponsorshipPublikationsfonds MLU-
dc.language.isoeng-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.subject.ddc540-
dc.titleA diazirine-modified membrane lipid to study peptide/lipid interactions : chances and challengeseng
dc.typeArticle-
local.versionTypepublishedVersion-
local.bibliographicCitation.journaltitleChemistry - a European journal-
local.bibliographicCitation.volume27-
local.bibliographicCitation.issue59-
local.bibliographicCitation.pagestart14586-
local.bibliographicCitation.pageend14593-
local.bibliographicCitation.publishernameWiley-VCH-
local.bibliographicCitation.publisherplaceWeinheim-
local.bibliographicCitation.doi10.1002/chem.202102048-
local.openaccesstrue-
local.accessrights.dnbfree-
Appears in Collections:Open Access Publikationen der MLU