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http://dx.doi.org/10.25673/73845
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DC Field | Value | Language |
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dc.contributor.author | Dorner, Julia | - |
dc.contributor.author | Korn, Patricia | - |
dc.contributor.author | Gruhle, Kai | - |
dc.contributor.author | Ramsbeck, Daniel | - |
dc.contributor.author | Garamus, Vasil M. | - |
dc.contributor.author | Lilie, Hauke | - |
dc.contributor.author | Meister, Annette | - |
dc.contributor.author | Schwieger, Christian | - |
dc.contributor.author | Ihling, Christian | - |
dc.contributor.author | Sinz, Andrea | - |
dc.contributor.author | Drescher, Simon | - |
dc.date.accessioned | 2022-03-08T12:21:48Z | - |
dc.date.available | 2022-03-08T12:21:48Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | https://opendata.uni-halle.de//handle/1981185920/75797 | - |
dc.identifier.uri | http://dx.doi.org/10.25673/73845 | - |
dc.description.abstract | Although incorporation of photo-activatable lipids into membranes potentially opens up novel avenues for investigating interactions with proteins, the question of whether diazirine-modified lipids are suitable for such studies, remains under debate. Focusing on the potential for studying lipid/peptide interactions by cross-linking mass spectrometry (XL-MS), we developed a diazirine-modified lipid (DiazPC), and examined its behaviour in membranes incorporating the model α-helical peptide LAVA20. We observed an unexpected backfolding of the diazirine-containing stearoyl chain of the lipid. This surprising behaviour challenges the potential application of DiazPC for future XL-MS studies of peptide and protein/lipid interactions. The observations made for DiazPC most likely represent a general phenomenon for any type of membrane lipids with a polar moiety incorporated into the alkyl chain. Our finding is therefore of importance for future protein/lipid interaction studies relying on modified lipid probes. | eng |
dc.description.sponsorship | Publikationsfonds MLU | - |
dc.language.iso | eng | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject.ddc | 540 | - |
dc.title | A diazirine-modified membrane lipid to study peptide/lipid interactions : chances and challenges | eng |
dc.type | Article | - |
local.versionType | publishedVersion | - |
local.bibliographicCitation.journaltitle | Chemistry - a European journal | - |
local.bibliographicCitation.volume | 27 | - |
local.bibliographicCitation.issue | 59 | - |
local.bibliographicCitation.pagestart | 14586 | - |
local.bibliographicCitation.pageend | 14593 | - |
local.bibliographicCitation.publishername | Wiley-VCH | - |
local.bibliographicCitation.publisherplace | Weinheim | - |
local.bibliographicCitation.doi | 10.1002/chem.202102048 | - |
local.openaccess | true | - |
local.accessrights.dnb | free | - |
Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
File | Description | Size | Format | |
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Chemistry A European J - 2021 - Dorner - A Diazirine‐Modified Membrane Lipid to Study Peptide Lipid Interactions Chances.pdf | 1.96 MB | Adobe PDF | View/Open |