Please use this identifier to cite or link to this item:
Title: Targeted introduction of heritable point mutations into the plant mitochondrial genome
Author(s): Forner, Joachim
Kleinschmidt, Dennis
Meyer, Etienne H.
Fischer, Axel
Morbitzer, Robert
Lahaye, Thomas
Schöttler, Mark A.
Bock, Ralph
Issue Date: 2022
Type: Article
Language: English
Abstract: The development of technologies for the genetic manipulation of mitochondrial genomes remains a major challenge. Here we report a method for the targeted introduction of mutations into plant mitochondrial DNA (mtDNA) that we refer to as transcription activator-like effector nuclease (TALEN) gene-drive mutagenesis (GDM), or TALEN-GDM. The method combines TALEN-induced site-specific cleavage of the mtDNA with selection for mutations that confer resistance to the TALEN cut. Applying TALEN-GDM to the tobacco mitochondrial nad9 gene, we isolated a large set of mutants carrying single amino acid substitutions in the Nad9 protein. The mutants could be purified to homochondriomy and stably inherited their edited mtDNA in the expected maternal fashion. TALEN-GDM induces both transitions and transversions, and can access most nucleotide positions within the TALEN binding site. Our work provides an efficient method for targeted mitochondrial genome editing that produces genetically stable, homochondriomic and fertile plants with specific point mutations in their mtDNA.
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Sponsor/Funder: Publikationsfonds MLU
Journal Title: Nature plants
Publisher: Nature Publ. Group
Publisher Place: London
Volume: 8
Original Publication: 10.1038/s41477-022-01108-y
Page Start: 245
Page End: 256
Appears in Collections:Open Access Publikationen der MLU

Files in This Item:
File Description SizeFormat 
s41477-022-01108-y.pdf10.12 MBAdobe PDFThumbnail