Please use this identifier to cite or link to this item:
http://dx.doi.org/10.25673/86343
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DC Field | Value | Language |
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dc.contributor.author | Paschold, Lisa | - |
dc.contributor.author | Klee, Bianca | - |
dc.contributor.author | Gottschick, Cornelia | - |
dc.contributor.author | Willscher, Edith | - |
dc.contributor.author | Diexer, Sophie | - |
dc.contributor.author | Schultheiß, Christoph | - |
dc.contributor.author | Simnica, Donjete | - |
dc.contributor.author | Sedding, Daniel | - |
dc.contributor.author | Girndt, Matthias | - |
dc.contributor.author | Gekle, Michael | - |
dc.contributor.author | Mikolajczyk, Rafael | - |
dc.contributor.author | Binder, Mascha | - |
dc.date.accessioned | 2022-07-12T07:05:38Z | - |
dc.date.available | 2022-07-12T07:05:38Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | https://opendata.uni-halle.de//handle/1981185920/88296 | - |
dc.identifier.uri | http://dx.doi.org/10.25673/86343 | - |
dc.description.abstract | The COVID-19 pandemic shows that vaccination strategies building on an ancestral viral strain need to be optimized for the control of potentially emerging viral variants. Therefore, aiming at strong B cell somatic hypermutation to increase antibody affinity to the ancestral strain - not only at high antibody titers - is a priority when utilizing vaccines that are not targeted at individual variants since high affinity may offer some flexibility to compensate for strain-individual mutations. Here, we developed a next-generation sequencing based SARS-CoV-2 B cell tracking protocol to rapidly determine the level of immunoglobulin somatic hypermutation at distinct points during the immunization period. The percentage of somatically hypermutated B cells in the SARS-CoV-2 specific repertoire was low after the primary vaccination series, evolved further over months and increased steeply after boosting. The third vaccination mobilized not only naïve, but also antigen-experienced B cell clones into further rapid somatic hypermutation trajectories indicating increased affinity. Together, the strongly mutated post-booster repertoires and antibodies deriving from this may explain why the third, but not the primary vaccination series, offers some protection against immune-escape variants such as Omicron B.1.1.529. | eng |
dc.description.sponsorship | Publikationsfonds MLU | - |
dc.language.iso | eng | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject.ddc | 611 | - |
dc.title | Rapid hypermutation B cell trajectory recruits previously primed B cells upon third SARS-Cov-2 mRNA vaccination | eng |
dc.type | Article | - |
local.versionType | publishedVersion | - |
local.bibliographicCitation.journaltitle | Frontiers in immunology | - |
local.bibliographicCitation.volume | 13 | - |
local.bibliographicCitation.publishername | Frontiers Media | - |
local.bibliographicCitation.publisherplace | Lausanne | - |
local.bibliographicCitation.doi | 10.3389/fimmu.2022.876306 | - |
local.openaccess | true | - |
local.accessrights.dnb | free | - |
Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
File | Description | Size | Format | |
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fimmu-13-876306.pdf | 7.25 MB | Adobe PDF | ![]() View/Open |