Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/101686
Title: The vacuolar transporter LaMTP8.1 detoxifies manganese in leaves of Lupinus albus
Author(s): Olt, PhilippLook up in the Integrated Authority File of the German National Library
Alejandro-Martinez, Santiago
Fermum, Johann
Ramos, Edith
Peiter, EdgarLook up in the Integrated Authority File of the German National Library
Ludewig, UweLook up in the Integrated Authority File of the German National Library
Issue Date: 2022
Type: Article
Language: English
Abstract: Manganese (Mn) is an essential microelement, but overaccumulation is harmful to many plant species. Most plants have similar minimal Mn requirements, but the tolerance to elevated Mn varies considerably. Mobilization of phosphate (P) by plant roots leads to increased Mn uptake, and shoot Mn levels have been reported to serve as an indicator for P mobilization efficiency in the presence of P deficiency. White lupin (Lupinus albus L.) mobilizes P and Mn with outstanding efficiency due to the formation of determinate cluster roots that release carboxylates. The high Mn tolerance of L. albus goes along with shoot Mn accumulation, but the molecular basis of this detoxification mechanism has been unknown. In this study, we identify LaMTP8.1 as the transporter mediating vacuolar sequestration of Mn in the shoot of white lupin. The function of Mn transport was demonstrated by yeast complementation analysis, in which LaMTP8.1 detoxified Mn in pmr1∆ mutant cells upon elevated Mn supply. In addition, LaMTP8.1 also functioned as an iron (Fe) transporter in yeast assays. The expression of LaMTP8.1 was particularly high in old leaves under high Mn stress. However, low P availability per se did not result in transcriptional upregulation of LaMTP8.1. Moreover, LaMTP8.1 expression was strongly upregulated under Fe deficiency, where it was accompanied by Mn accumulation, indicating a role in the interaction of these micronutrients in L. albus. In conclusion, the tonoplast-localized Mn transporter LaMTP8.1 mediates Mn detoxification in leaf vacuoles, providing a mechanistic explanation for the high Mn accumulation and Mn tolerance in this species.
URI: https://opendata.uni-halle.de//handle/1981185920/103633
http://dx.doi.org/10.25673/101686
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Journal Title: Physiologia plantarum
Publisher: Wiley-Blackwell
Publisher Place: Oxford [u.a.]
Volume: 174
Issue: 6
Original Publication: 10.1111/ppl.13807
Page Start: 1
Page End: 11
Appears in Collections:Open Access Publikationen der MLU