Bitte benutzen Sie diese Kennung, um auf die Ressource zu verweisen:
http://dx.doi.org/10.25673/102984
Titel: | Early stage UV-B induced molecular modifications of human eye lens γD-crystallin |
Autor(en): | Weininger, Susanne![]() Neudorf, Malte Gröger, Stefan Plato, Eric Broneske, Robert Saalwächter, Kay ![]() Weininger, Ulrich ![]() Balbach, Jochen ![]() |
Erscheinungsdatum: | 2023 |
Art: | Artikel |
Sprache: | Englisch |
Zusammenfassung: | In the human eye lenses, the crystallin proteins facilitate transparency, light refraction, as well as UV light protection. A deregulated balanced interplay between α-, β-, and γ-crystallin can cause cataract. γD-crystallin (hγD) is involved in the energy dissipation of absorbed UV light by energy transfer between aromatic side chains. Early UV-B induced damage of hγD with molecular resolution is studied by solution NMR and fluorescence spectroscopy. hγD modifications are restricted to Tyr 17 and Tyr 29 in the N-terminal domain, where a local unfolding of the hydrophobic core is observed. None of the tryptophan residues assisting fluorescence energy transfer is modified and hγD is remained soluble over month. Investigating isotope-labeled hγD surrounded by eye lens extracts from cataract patients reveals very week interactions of solvent-exposed side chains in the C-terminal hγD domain and some remaining photoprotective properties of the extracts. Hereditary E107A hγD found in the eye lens core of infants developing cataract shows under the here used conditions a thermodynamic stability comparable to the wild type but an increased sensitivity toward UV-B irradiation. |
URI: | https://opendata.uni-halle.de//handle/1981185920/104937 http://dx.doi.org/10.25673/102984 |
Open-Access: | ![]() |
Nutzungslizenz: | ![]() |
Journal Titel: | Macromolecular bioscience |
Verlag: | Wiley-VCH |
Verlagsort: | Weinheim |
Originalveröffentlichung: | 10.1002/mabi.202200526 |
Enthalten in den Sammlungen: | Open Access Publikationen der MLU |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
---|---|---|---|---|
Macromolecular Bioscience - 2023 - Weininger - Early Stage UV‐B Induced Molecular Modifications of Human Eye Lens .pdf | 2.09 MB | Adobe PDF | ![]() Öffnen/Anzeigen |