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http://dx.doi.org/10.25673/110254
Titel: | Development of a rapid and specific MALDI-TOF mass spectrometric assay for SARS-CoV-2 detection |
Autor(en): | Kollhoff, Lydia Kipping, Marc ![]() Rauh, Manfred Ceglarek, Uta ![]() Barka, Günes Barka, Frederik Sinz, Andrea ![]() |
Erscheinungsdatum: | 2023 |
Art: | Artikel |
Sprache: | Englisch |
Zusammenfassung: | We have developed a rapid and highly specific assay for detecting and monitoring SARS-CoV-2 infections by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). As MALDI-TOF mass spectrometers are available in a clinical setting, our assay has the potential to serve as alternative to the commonly used reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). Sample preparation prior to MALDI-TOF-MS involves the tryptic digestion of SARS-CoV-2 proteins, followed by an enrichment of virus-specific peptides from SARS-CoV-2 nucleoprotein via magnetic antibody beads. Our MALDI-TOF-MS method allows the detection of SARS-CoV-2 nucleoprotein in sample collection medium as low as 8 amol/µl. MALDI-TOF mass spectra are obtained in just a few seconds, which makes our MS-based assay suitable for a high-throughput screening of SARS-CoV-2 in healthcare facilities in addition to PCR. Due to the specific detection of virus peptides, different SARS-CoV-2 variants are readily distinguished from each other. Specifically, we show that our MALDI-TOF-MS assay discriminates SARS-CoV-2 strain B.1.617.2 “delta variant” from all other variants in patients’ samples, making our method highly valuable to monitor the emergence of new virus variants. |
URI: | https://opendata.uni-halle.de//handle/1981185920/112209 http://dx.doi.org/10.25673/110254 |
Open-Access: | ![]() |
Nutzungslizenz: | ![]() |
Journal Titel: | Clinical proteomics |
Verlag: | Humana Press |
Verlagsort: | Totowa, NJ |
Band: | 20 |
Heft: | 1 |
Originalveröffentlichung: | 10.1186/s12014-023-09415-y |
Enthalten in den Sammlungen: | Open Access Publikationen der MLU |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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s12014-023-09415-y.pdf | 1.3 MB | Adobe PDF | ![]() Öffnen/Anzeigen |