Increased stress levels in caged honeybee (Apis mellifera) (Hymenoptera: Apidae) workers

Abstract

Honeybees, Apis mellifera, usually live in large colonies consisting of thousands of individuals. Within the colony, workers interact with their social environment frequently. The large workforce, division of labour, and other features may promote the ecological success of honeybees. For decades, artificial mini colonies in cages within the laboratory have become the gold standard, especially in experiments related to toxicology, effects of pesticides and pathogens. Experiments using caged bees and full-sized colonies yielded contradictory results. Here, the effect of cage experiments on the stress level of individual bees is analysed. Two different stress response were targeted, the heat shock response and the mobilization of energetic resources. While no differences were found for varying group sizes of bees, very strong effects emerged by comparing caged workers with bees from natural colonies. Caged workers showed increased levels of hsp expression and reduced haemolymph titres for trehalose, the energy storage sugar. These results reveal that the lack of the social environment (e.g., lack of queen, lack of sufficient group size) induce stress in caged bees, which might act synergistically when bees are challenged by additional stressors (e.g., pesticides, pathogens) resulting in higher mortality than observed under field conditions.