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http://dx.doi.org/10.25673/111306
Titel: | The structural principles underlying molybdenum insertase complex assembly |
Autor(en): | Hassan, Ahmed H. Ihling, Christian Iacobucci, Claudio Kastritis, Panagiotis L. Sinz, Andrea Kruse, Tobias |
Erscheinungsdatum: | 2023 |
Art: | Artikel |
Sprache: | Englisch |
Zusammenfassung: | Within the cell, the trace element molybdenum (Mo) is only biologically active when complexed either within the nitrogenase-specific FeMo cofactor or within the molybdenum cofactor (Moco). Moco consists of an organic part, called molybdopterin (MPT) and an inorganic part, that is, the Mo-center. The enzyme which catalyzes the Mo-center formation is the molybdenum insertase (Mo-insertase). Mo-insertases consist of two functional domains called G- and E-domain. The G-domain catalyzes the formation of adenylated MPT (MPT-AMP), which is the substrate for the E-domain, that catalyzes the actual molybdate insertion reaction. Though the functions of E- and G-domain have been elucidated to great structural and mechanistic detail, their combined function is poorly characterized. In this work, we describe a structural model of the eukaryotic Mo-insertase Cnx1 complex that was generated based on cross-linking mass spectrometry combined with computational modeling. We revealed Cnx1 to form an asymmetric hexameric complex which allows the E- and G-domain active sites to align in a catalytic productive orientation toward each other. |
URI: | https://opendata.uni-halle.de//handle/1981185920/113260 http://dx.doi.org/10.25673/111306 |
Open-Access: | Open-Access-Publikation |
Nutzungslizenz: | (CC BY-NC-ND 4.0) Creative Commons Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
Journal Titel: | Protein science |
Verlag: | Wiley |
Verlagsort: | Hoboken, NJ |
Band: | 32 |
Heft: | 9 |
Originalveröffentlichung: | 10.1002/pro.4753 |
Enthalten in den Sammlungen: | Open Access Publikationen der MLU |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Protein Science - 2023 - Hassan - The structural principles underlying molybdenum insertase complex assembly.pdf | 5.24 MB | Adobe PDF | Öffnen/Anzeigen |