Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/111310
Title: A conserved microtubule-binding region in Xanthomonas XopL is indispensable for induced plant cell death reactions
Author(s): Ortmann, Simon
Marx, Jolina
Lampe, ChristinaLook up in the Integrated Authority File of the German National Library
Handrick, VinzenzLook up in the Integrated Authority File of the German National Library
Ehnert, Tim-Martin
Zinecker, SarahLook up in the Integrated Authority File of the German National Library
Reimers, MatthiasLook up in the Integrated Authority File of the German National Library
Bonas, UllaLook up in the Integrated Authority File of the German National Library
Erickson, JessicaLook up in the Integrated Authority File of the German National Library
Issue Date: 2023
Type: Article
Language: English
Abstract: Pathogenic Xanthomonas bacteria cause disease on more than 400 plant species. These Gram-negative bacteria utilize the type III secretion system to inject type III effector proteins (T3Es) directly into the plant cell cytosol where they can manipulate plant pathways to promote virulence. The host range of a given Xanthomonas species is limited, and T3E repertoires are specialized during interactions with specific plant species. Some effectors, however, are retained across most strains, such as Xanthomonas Outer Protein L (XopL). As an ‘ancestral’ effector, XopL contributes to the virulence of multiple xanthomonads, infecting diverse plant species. XopL homologs harbor a combination of a leucine-rich-repeat (LRR) domain and an XL-box which has E3 ligase activity. Despite similar domain structure there is evidence to suggest that XopL function has diverged, exemplified by the finding that XopLs expressed in plants often display bacterial species-dependent differences in their sub-cellular localization and plant cell death reactions. We found that XopL from X. euvesicatoria (XopLXe) directly associates with plant microtubules (MTs) and causes strong cell death in agroinfection assays in N. benthamiana. Localization of XopLXe homologs from three additional Xanthomonas species, of diverse infection strategy and plant host, revealed that the distantly related X. campestris pv. campestris harbors a XopL (XopLXcc) that fails to localize to MTs and to cause plant cell death. Comparative sequence analyses of MT-binding XopLs and XopLXcc identified a proline-rich-region (PRR)/α-helical region important for MT localization. Functional analyses of XopLXe truncations and amino acid exchanges within the PRR suggest that MT-localized XopL activity is required for plant cell death reactions. This study exemplifies how the study of a T3E within the context of a genus rather than a single species can shed light on how effector localization is linked to biochemical activity.
URI: https://opendata.uni-halle.de//handle/1981185920/113264
http://dx.doi.org/10.25673/111310
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Journal Title: PLoS pathogens
Publisher: PLoS
Publisher Place: Lawrence, Kan.
Volume: 19
Issue: 8
Original Publication: 10.1371/journal.ppat.1011263
Page Start: 1
Page End: 32
Appears in Collections:Open Access Publikationen der MLU

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