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http://dx.doi.org/10.25673/115074| Title: | Enabling cryo-EM density interpretation from yeast native cell extracts by proteomics data and AlphaFold structures |
| Author(s): | Tüting, Christian Schmidt, Lisa Skalidis, Ioannis Sinz, Andrea Kastritis, Panagiotis L. |
| Issue Date: | 2023 |
| Type: | Article |
| Language: | English |
| Abstract: | Inthecellularcontext,proteinsparticipateincommunitiestoperformtheirfunction.Thedetectionandidentificationofthesecommunitiesaswellasin-communityinter-actionshaslongbeenthesubjectofinvestigation,mainlythroughproteomicsanalysiswithmassspectrometry.Withtheadventofcryogenicelectronmicroscopyandthe“resolutionrevolution,”theirvisualizationhasrecentlybeenmadepossible,evenincomplex, native samples. The advances in both fields have resulted in the genera-tionoflargeamountsofdata,whoseanalysisrequiresadvancedcomputation,oftenemployingmachinelearningapproachestoreachthedesiredoutcome.Inthiswork,wefirstperformedarobustproteomicsanalysisofmassspectrometry(MS)dataderivedfromayeastnativecellextractandusedthisinformationtoidentifyproteincommu-nitiesandinter-proteininteractions.Cryo-EManalysisofthecellextractprovidedareconstructionofabiomoleculeatmediumresolution(∼8Å(FSC=0.143)).Utiliz-ingMS-derivedproteomicsdataandsystematicfittingofAlphaFold-predictedatomicmodels, this density was assigned to the 2.6 MDa complex of yeast fatty acid syn-thase.OurproposedworkflowidentifiesproteincomplexesinnativecellextractsfromSaccharomyces cerevisiaeby combining proteomics, cryo-EM, and AI-guided proteinstructureprediction. |
| URI: | https://opendata.uni-halle.de//handle/1981185920/117030 http://dx.doi.org/10.25673/115074 |
| Open Access: |  Open access publication |
| License: |  (CC BY 4.0) Creative Commons Attribution 4.0 |
| Journal Title: | Proteomics |
| Publisher: | Wiley VCH |
| Publisher Place: | Weinheim |
| Volume: | 23 |
| Issue: | 17 |
| Original Publication: | 10.1002/pmic.202200096 |
| Page Start: | 1 |
| Page End: | 10 |
| Appears in Collections: | Open Access Publikationen der MLU |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Proteomics - 2023 - Tüting - Enabling cryo‐EM density interpretation from yeast native cell extracts by proteomics data and.pdf | 1.96 MB | Adobe PDF |  View/Open |