Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/118288
Title: Cantharidin and sodium fluoride attenuate the negative inotropic effect of the A₁-adenosine receptor agonist N⁶-(R)-phenylisopropyl adenosine in isolated human atria
Author(s): Schwarz, Rebecca LiaLook up in the Integrated Authority File of the German National Library
Hofmann, BrittLook up in the Integrated Authority File of the German National Library
Gergs, UlrichLook up in the Integrated Authority File of the German National Library
Neumann, JoachimLook up in the Integrated Authority File of the German National Library
Issue Date: 2025
Type: Article
Language: English
Abstract: Cantharidin and sodium fluoride inhibit the activity of serine/threonine protein phosphatases 1 (PP1) and 2A (PP2A) and increase the force of contraction in human atrial preparations. R-phenylisopropyl adenosine (R-PIA) acts as an agonist at A₁-adenosine receptors. R-PIA exerts a negative inotropic effect on human atria. The effect of R-PIA - and its various manifestations - are currently explained as a function of the inhibition of sarcolemmal adenylyl cyclase activity and/or opening of sarcolemmal potassium channels. We hypothesise that cantharidin and sodium fluoride may attenuate the negative inotropic effect of R-PIA. During open heart surgery, trabeculae carneae from the right atrium were obtained for human atrial preparations (HAPs). These trabeculae were mounted in organ baths and electrically stimulated at 1 Hz. Furthermore, we studied isolated electrically stimulated left atrial (LA) preparations from female wild-type mice (CD1). The force of contraction was recorded under isometric conditions. R-PIA (1 µM) exerted a rapid negative inotropic effect in the HAPs and mice LA preparations. These negative inotropic effects of R-PIA were attenuated by pre-incubation for 30 min with 100-µM cantharidin in HAPs, but not in mice LA preparations. Adenosine signals via A₁ receptors in a species-specific pathway in mammalian atria. We postulate that R-PIA, at least in part, exerts a negative inotropic effect via activation of serine/threonine phosphatases in the human atrium.
URI: https://opendata.uni-halle.de//handle/1981185920/120247
http://dx.doi.org/10.25673/118288
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Journal Title: Naunyn-Schmiedeberg's archives of pharmacology
Publisher: Springer
Publisher Place: Berlin
Volume: 398
Issue: 2
Original Publication: 10.1007/s00210-024-03402-2
Page Start: 1961
Page End: 1971
Appears in Collections:Open Access Publikationen der MLU

Files in This Item:
File Description SizeFormat 
s00210-024-03402-2.pdf1.28 MBAdobe PDFThumbnail
View/Open