Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/118668
Title: From photon to product : development and quantitative assessment of photosynthesis-driven oxygenase biocatalysis with cyanobacteria
Author(s): Tüllinghoff, AdrianLook up in the Integrated Authority File of the German National Library
Referee(s): Bühler, BrunoLook up in the Integrated Authority File of the German National Library
Pietzsch, MarkusLook up in the Integrated Authority File of the German National Library
Zedler, JulieLook up in the Integrated Authority File of the German National Library
Granting Institution: Martin-Luther-Universität Halle-Wittenberg
Issue Date: 2024
Extent: 1 Online-Ressource (X, 147 Seiten)
Type: HochschulschriftLook up in the Integrated Authority File of the German National Library
Type: PhDThesis
Exam Date: 2024-05-23
Language: English
URN: urn:nbn:de:gbv:3:4-1981185920-1206267
Abstract: Cyanobacteria are promising whole-cell biocatalysts for sustainable production light-driven processes. Heterologous oxygenases can profit from photosynthesis-derived reduction equivalents and O2 for biocatalysis. Here, a Cytochrome P450 monooxygenase and a Baeyer-Villiger monooxygenase were established, characterized, and optimized on the genetic and reaction level. With these model systems we investigated i) which electron routes can be used to couple heterologous redox reactions to photosynthesis, ii) how efficient electron transfer to heterologous redox reactions can be realized, and iii) at which rate photosynthetic electron supply can be tapped for production processes. The establishment of an in vivo enzyme cascade in a cyanobacterium marks another important progress, inter alia to overcome product inhibition. Combining biocatalyst and process engineering with a quantitative perspective on cyanobacteria is of significant interest for future developments in the field.
URI: https://opendata.uni-halle.de//handle/1981185920/120626
http://dx.doi.org/10.25673/118668
Open Access: Open access publication
License: (CC BY-NC 4.0) Creative Commons Attribution NonCommercial 4.0(CC BY-NC 4.0) Creative Commons Attribution NonCommercial 4.0
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