Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/38644
Title: Phase diagram for a lysyl-phosphatidylglycerol analogue in biomimetic mixed monolayers with phosphatidylglycerol : insights into the tunable properties of bacterial membranes
Author(s): Wölk, Christian
Youssef, Hala
Guttenberg, Thomas
Marbach, Helene
Vizcay-Barrena, Gema
Shen, Chen
Brezesinski, Gerald
Harvey, Richard D.
Issue Date: 2020
Type: Article
Language: English
Abstract: Ion pairing between the major phospholipids of the Staphylococcus aureus plasma membrane (phosphatidylglycerol – PG and lysyl-phosphatidylglycerol – LPG) confers resistance to antimicrobial peptides and other antibiotics. We developed 3adLPG, a stable synthetic analogue which can substitute for the highy-labile native LPG, in biophysical experiments examining the membrane-protecting role of lipid ion pairing, in S. aureus and other important bacteria. Here we examine the surface charge and lipid packing characteristics of synthetic biomimetic mixtures of DPPG and DP3adLPG in Langmuir monolayers, using a combination of complementary surface-probing techniques such as infrared reflection-absorption spectroscopy and grazing-incidence x-ray diffraction. The resultant phase diagram for the ion paired lipids sheds light on the mixing behavior of lipids in monolayer models of resistant phenotype bacterial membranes, and provides a platform for future biophysical studies.
URI: https://opendata.uni-halle.de//handle/1981185920/38890
http://dx.doi.org/10.25673/38644
Open Access: Open access publication
License: (CC BY-NC-ND 4.0) Creative Commons Attribution NonCommercial NoDerivatives 4.0(CC BY-NC-ND 4.0) Creative Commons Attribution NonCommercial NoDerivatives 4.0
Sponsor/Funder: Publikationsfonds MLU
Journal Title: ChemPhysChem
Publisher: Wiley-VCH Verl.
Publisher Place: Weinheim
Volume: 21
Issue: 8
Original Publication: 10.1002/cphc.202000026
Page Start: 702
Page End: 706
Appears in Collections:Open Access Publikationen der MLU

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