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Titel: Ionizable lipids based on branched fatty acids : an explorative study on Langmuir monolayers
Autor(en): Pawlowska, Dorota
Erdmann, Nicole
Folz, Manuela
Langner, AndreasIn der Gemeinsamen Normdatei der DNB nachschlagen
Dobner, Bodo
Wölk, ChristianIn der Gemeinsamen Normdatei der DNB nachschlagen
Brezesinski, GeraldIn der Gemeinsamen Normdatei der DNB nachschlagen
Erscheinungsdatum: 2024
Art: Artikel
Sprache: Englisch
Zusammenfassung: Ionizable lipids are a class of pharmaceutical excipients with a main application in lipid nanoparticles for nucleic acid delivery. New ionizable lipids are needed to tune characteristics of lipid-based nucleic acid delivery systems, e.g. stability, nucleic acid loading capacity and binding strength, as well as bio-distribution. Herein, we present the synthesis of three novel ionizable lipids as putative excipients for lipid-based nucleic acid delivery systems. Langmuir monolayer experiments with classical surface pressure/area isotherm evaluation were used to understand the self-assembly behavior of the lipids. Additional experiments with surface sensitive techniques, namely grazing incidence x-ray scattering and infrared reflection–absorption spectroscopy (IRRAS), were performed to understand structural characteristics of lipid associates. The latter technique was also used to investigate the nucleic acid binding process between DNA and the ionizable lipids. Finally, first transfection experiments with the novel lipids formulated as cationic liposomes were performed providing first efficacy data. Although the alkyl chain pattern was comparable for all three ionizable lipids, the results demonstrated that with increasing head-group size the DNA binding capacity changed and the alkyl chain fluidity was increased. The lipid with the lowest phase transition temperature and the smallest packing parameter showed the highest DNA transfer efficiency.
URI: https://opendata.uni-halle.de//handle/1981185920/118415
http://dx.doi.org/10.25673/116460
Open-Access: Open-Access-Publikation
Nutzungslizenz: (CC BY 4.0) Creative Commons Namensnennung 4.0 International(CC BY 4.0) Creative Commons Namensnennung 4.0 International
Journal Titel: European journal of pharmaceutics and biopharmaceutics
Verlag: Elsevier
Verlagsort: New York, NY [u.a.]
Band: 200
Originalveröffentlichung: 10.1016/j.ejpb.2024.114338
Enthalten in den Sammlungen:Open Access Publikationen der MLU

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