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Titel: Impact of siRNA-mediated Cofilin-1 knockdown and obesity associated microenvironment on the motility of natural killer cells
Autor(en): Griesler, BrunoIn der Gemeinsamen Normdatei der DNB nachschlagen
Hölzel, Marijke
Oswald, JanaIn der Gemeinsamen Normdatei der DNB nachschlagen
Fänder, Johannes
Fischer, Trutz-Eckhardt Uwe Hermann SiegfriedIn der Gemeinsamen Normdatei der DNB nachschlagen
Büttner, MaximilianIn der Gemeinsamen Normdatei der DNB nachschlagen
Quandt, DagmarIn der Gemeinsamen Normdatei der DNB nachschlagen
Bähr, InaIn der Gemeinsamen Normdatei der DNB nachschlagen
Jasinski-Bergner, SimonIn der Gemeinsamen Normdatei der DNB nachschlagen
Ivonne, Bazwinsky-Wutschke
Kielstein, HeikeIn der Gemeinsamen Normdatei der DNB nachschlagen
Erscheinungsdatum: 2024
Art: Artikel
Sprache: Englisch
Zusammenfassung: The anti-tumor capacity of natural killer (NK) cells heavily relies on their ability to migrate towards their target cells. This process is based on dynamic actinrearrangement, so-called actin treadmilling, andis tightly regulated by proteins such as cofilin-1. The aim of the present study was to identify the role of cofilin-1 (CFL-1) in the migratory behavior of NK cells and to investigate a possible impact of an obesity-associated micromilieu on these cells, as it is known that obesity correlates with various impaired NK cell functions. CFL-1 was knocked-down via transfection of NK-92 cells with respective siRNAs. Obesity associated micromilieu was mimicked by incubation of NK-92 cells with adipocyte-conditioned medium from human preadipocyte SGBS cells or leptin. Effects on CFL-1 levels, the degree of phosphorylation to the inactive pCFL-1 as well as NK-92 cell motility were analyzed. Surprisingly, siRNA-mediated CFL-1 knockdown led to a significant increase of migration, as determined by enhanced velocity and accumulated distance of migration. No effect on CFL-1 nor pCFL-1 expression levels, proportion of phosphorylation and cell migratory behavior could be demonstrated under the influence of an obesity-associated microenvironment. In conclusion, the results indicate a significant effect of a CFL-1 knockdown on NK cell motility.
URI: https://opendata.uni-halle.de//handle/1981185920/119605
http://dx.doi.org/10.25673/117646
Open-Access: Open-Access-Publikation
Nutzungslizenz: (CC BY-NC-ND 4.0) Creative Commons Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International(CC BY-NC-ND 4.0) Creative Commons Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International
Journal Titel: Immunological investigations
Verlag: Taylor & Francis Group
Verlagsort: London [u.a.]
Band: 53
Heft: 5
Originalveröffentlichung: 10.1080/08820139.2024.2327327
Seitenanfang: 713
Seitenende: 729
Enthalten in den Sammlungen:Open Access Publikationen der MLU