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Title: Dynamics and genetic regulation of leaf nutrient concentration in barley based on hyperspectral imaging and machine learning
Author(s): Grieco, Michele
Schmidt, Maria
Warnemünde, Sebastian
Backhaus, Andreas
Klück, Hans-Christian
Garibay, Adriana
Tandrón Moya, Yudelsy Antonia
Jozefowicz, Anna Maria
Mock, Hans-Peter
Seiffert, Udo
Maurer, Andreas
Pillen, Klaus
Issue Date: 2022
Type: Article
Language: English
Abstract: Biofortification, the enrichment of nutrients in crop plants, is of increasing importance to improve human health. The wild barley nested association mapping (NAM) population HEB-25 was developed to improve agronomic traits including nutrient concentration. Here, we evaluated the potential of high-throughput hyperspectral imaging in HEB-25 to predict leaf concentration of 15 mineral nutrients, sampled from two field experiments and four developmental stages. Particularly accurate predictions were obtained by partial least squares regression (PLS) modeling of leaf concentrations for N, P and K reaching coefficients of determination of 0.90, 0.75 and 0.89, respectively. We recognized nutrient-specific patterns of variation of leaf nutrient concentration between developmental stages. A number of quantitative trait loci (QTL) associated with the simultaneous expression of leaf nutrients were detected, indicating their potential co-regulation in barley. For example, the wild barley allele of QTL-4H-1 simultaneously increased leaf concentration of N, P, K and Cu. Similar effects of the same QTL were previously reported for nutrient concentrations in grains, supporting a potential parallel regulation of N, P, K and Cu in leaves and grains of HEB-25. Our study provides a new approach for nutrient assessment in large-scale field experiments to ultimately select genes and genotypes supporting plant biofortification.
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Sponsor/Funder: Publikationsfonds MLU
Journal Title: Plant science
Publisher: Elsevier Science
Publisher Place: Amsterdam [u.a.]
Volume: 315
Original Publication: 10.1016/j.plantsci.2021.111123
Appears in Collections:Open Access Publikationen der MLU

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