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http://dx.doi.org/10.25673/103280
Titel: | The role of IL-13 and IL-4 in adipose tissue fibrosis |
Autor(en): | Arndt, Lilli Lindhorst, Andreas ![]() Neugebauer, Julia ![]() Hoffmann, Anne Hobusch, Constance Alexakē, Basileia-Ismēnē ![]() Ghosh, Adhideb ![]() Blüher, Matthias ![]() Wolfrum, Christian ![]() Glaß, Markus Gericke, Martin ![]() |
Erscheinungsdatum: | 2023 |
Art: | Artikel |
Sprache: | Englisch |
Zusammenfassung: | White adipose tissue (WAT) fibrosis, characterized by an excess of extracellular (ECM) matrix components, is strongly associated with WAT inflammation and dysfunction due to obesity. Interleukin (IL)-13 and IL-4 were recently identified as critical mediators in the pathogenesis of fibrotic diseases. However, their role in WAT fibrosis is still ill-defined. We therefore established an ex vivo WAT organotypic culture system and demonstrated an upregulation of fibrosis-related genes and an increase of α-smooth muscle actin (αSMA) and fibronectin abundance upon dose-dependent stimulation with IL-13/IL-4. These fibrotic effects were lost in WAT lacking il4ra, which encodes for the underlying receptor controlling this process. Adipose tissue macrophages were found to play a key role in mediating IL-13/IL-4 effects in WAT fibrosis as their depletion through clodronate dramatically decreased the fibrotic phenotype. IL-4-induced WAT fibrosis was partly confirmed in mice injected intraperitoneally with IL-4. Furthermore, gene correlation analyses of human WAT samples revealed a strong positive correlation of fibrosis markers with IL-13/IL-4 receptors, whereas IL13 and IL4 correlations failed to confirm this association. In conclusion, IL-13 and IL-4 can induce WAT fibrosis ex vivo and partly in vivo, but their role in human WAT remains to be further elucidated. |
URI: | https://opendata.uni-halle.de//handle/1981185920/105232 http://dx.doi.org/10.25673/103280 |
Open-Access: | ![]() |
Nutzungslizenz: | ![]() |
Journal Titel: | International journal of molecular sciences |
Verlag: | Molecular Diversity Preservation International |
Verlagsort: | Basel |
Band: | 24 |
Heft: | 6 |
Originalveröffentlichung: | 10.3390/ijms24065672 |
Seitenanfang: | 1 |
Seitenende: | 19 |
Enthalten in den Sammlungen: | Open Access Publikationen der MLU |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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ijms-24-05672.pdf | 2.68 MB | Adobe PDF | ![]() Öffnen/Anzeigen |