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http://dx.doi.org/10.25673/113661
Titel: | Identification and characterization of the anti-viral interferon lambda 3 as direct target of the Epstein-Barr virus microRNA-BART7-3p |
Autor(en): | Blümke, Juliane Bauer, Marcus Vaxevanis, Christoforos Wilfer, Andreas Mandelboim, Ofer Wickenhauser, Claudia Seliger, Barbara ![]() Jasinski-Bergner, Simon ![]() |
Erscheinungsdatum: | 2023 |
Art: | Artikel |
Sprache: | Englisch |
Zusammenfassung: | The human Epstein–Barr virus (EBV), as a member of the human γ herpes viruses (HHV), is known to be linked with distinct tumor types. It is a double-stranded DNA virus and its genome encodes among others for 48 different microRNAs (miRs). Current research demonstrated a strong involvement of certain EBV-miRs in molecular immune evasion mechanisms of infected cells by, e.g., the disruption of human leukocyte antigen (HLA) class Ia and NKG2D functions. To determine novel targets of EBV-miRs involved in immune surveillance, ebv-miR-BART7-3p, an EBV-encoded miR with high expression levels during the different lytic and latent EBV life cycle phases, was overexpressed in human HEK293T cells. Using a cDNA microarray-based comparative analysis, 234 (229 downregulated and 5 upregulated) deregulated human transcripts were identified in ebv-miR-BART7-3p transfectants, which were mainly involved in cellular processes and molecular binding. A statistically significant downregulation of the anti-proliferative and tumor-suppressive hsa-miR-34A and the anti-viral interferon lambda (IFNL)3 mRNA was found. The ebv-miR-BART7-3p-mediated downregulation of IFNL3 expression was due to a direct interaction with the IFNL3 3’-untranslated region (UTR) as determined by luciferase reporter gene assays including the identification of the accurate ebv-miR-BART7-3p binding site. The effect of ebv-miR-BART7-3p on the IFNL3 expression was validated both in human cell lines in vitro and in human tissue specimen with known EBV status. These results expand the current knowledge of EBV-encoded miRs and their role in immune evasion, pathogenesis and malignant transformation. |
URI: | https://opendata.uni-halle.de//handle/1981185920/115616 http://dx.doi.org/10.25673/113661 |
Open-Access: | ![]() |
Nutzungslizenz: | ![]() |
Journal Titel: | OncoImmunology |
Verlag: | Taylor & Franics |
Verlagsort: | Abingdon |
Band: | 12 |
Heft: | 1 |
Originalveröffentlichung: | 10.1080/2162402x.2023.2284483 |
Seitenanfang: | 1 |
Seitenende: | 11 |
Enthalten in den Sammlungen: | Open Access Publikationen der MLU |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Identification and characterization of the anti-viral interferon lambda 3 as direct target of the Epstein-Barr virus microRNA-BART7-3p.pdf | 3.4 MB | Adobe PDF | ![]() Öffnen/Anzeigen |