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http://dx.doi.org/10.25673/60207
Titel: | A rapid and reliable liquid chromatography/mass spectrometry method for SARS-CoV-2 analysis from gargle solutions and saliva |
Autor(en): | Kipping, Marc Tänzler, Dirk Sinz, Andrea |
Erscheinungsdatum: | 2021 |
Art: | Artikel |
Sprache: | Englisch |
Zusammenfassung: | We describe a rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the direct detection and quantitation of SARS-CoV-2 nucleoprotein in gargle solutions and saliva. The method is based on a multiple-reaction monitoring (MRM) mass spectrometry approach with a total cycle time of 5 min per analysis and allows the detection and accurate quantitation of SARS-CoV-2 nucleoprotein as low as 500 amol/μL. We improved the sample preparation protocol of our recent piloting SARS-CoV-2 LC-MS study regarding sensitivity, reproducibility, and compatibility with a complementary reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis of the same sample. The aim of this work is to promote diagnostic tools that allow identifying and monitoring SARS-CoV-2 infections by LC-MS/MS methods in a routine clinical environment. |
URI: | https://opendata.uni-halle.de//handle/1981185920/62158 http://dx.doi.org/10.25673/60207 |
Open-Access: | ![]() |
Nutzungslizenz: | ![]() |
Sponsor/Geldgeber: | Publikationsfonds MLU |
Journal Titel: | Analytical and Bioanalytical Chemistry |
Verlag: | Springer |
Verlagsort: | Berlin |
Band: | 413 |
Originalveröffentlichung: | 10.1007/s00216-021-03614-y |
Seitenanfang: | 6503 |
Seitenende: | 6511 |
Enthalten in den Sammlungen: | Open Access Publikationen der MLU |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Kipping2021_Article_ARapidAndReliableLiquidChromat.pdf | 802.48 kB | Adobe PDF | ![]() Öffnen/Anzeigen |