Please use this identifier to cite or link to this item: http://dx.doi.org/10.25673/86343
Title: Rapid hypermutation B cell trajectory recruits previously primed B cells upon third SARS-Cov-2 mRNA vaccination
Author(s): Paschold, Lisa
Klee, Bianca
Gottschick, Cornelia
Willscher, Edith
Diexer, Sophie
Schultheiß, Christoph
Simnica, Donjete
Sedding, Daniel
Girndt, Matthias
Gekle, Michael
Mikolajczyk, Rafael
Binder, Mascha
Issue Date: 2022
Type: Article
Language: English
Abstract: The COVID-19 pandemic shows that vaccination strategies building on an ancestral viral strain need to be optimized for the control of potentially emerging viral variants. Therefore, aiming at strong B cell somatic hypermutation to increase antibody affinity to the ancestral strain - not only at high antibody titers - is a priority when utilizing vaccines that are not targeted at individual variants since high affinity may offer some flexibility to compensate for strain-individual mutations. Here, we developed a next-generation sequencing based SARS-CoV-2 B cell tracking protocol to rapidly determine the level of immunoglobulin somatic hypermutation at distinct points during the immunization period. The percentage of somatically hypermutated B cells in the SARS-CoV-2 specific repertoire was low after the primary vaccination series, evolved further over months and increased steeply after boosting. The third vaccination mobilized not only naïve, but also antigen-experienced B cell clones into further rapid somatic hypermutation trajectories indicating increased affinity. Together, the strongly mutated post-booster repertoires and antibodies deriving from this may explain why the third, but not the primary vaccination series, offers some protection against immune-escape variants such as Omicron B.1.1.529.
URI: https://opendata.uni-halle.de//handle/1981185920/88296
http://dx.doi.org/10.25673/86343
Open Access: Open access publication
License: (CC BY 4.0) Creative Commons Attribution 4.0(CC BY 4.0) Creative Commons Attribution 4.0
Sponsor/Funder: Publikationsfonds MLU
Journal Title: Frontiers in immunology
Publisher: Frontiers Media
Publisher Place: Lausanne
Volume: 13
Original Publication: 10.3389/fimmu.2022.876306
Appears in Collections:Open Access Publikationen der MLU

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