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http://dx.doi.org/10.25673/86343
Titel: | Rapid hypermutation B cell trajectory recruits previously primed B cells upon third SARS-Cov-2 mRNA vaccination |
Autor(en): | Paschold, Lisa Klee, Bianca Gottschick, Cornelia Willscher, Edith Diexer, Sophie Schultheiß, Christoph Simnica, Donjete Sedding, Daniel Girndt, Matthias Gekle, Michael Mikolajczyk, Rafael Binder, Mascha |
Erscheinungsdatum: | 2022 |
Art: | Artikel |
Sprache: | Englisch |
Zusammenfassung: | The COVID-19 pandemic shows that vaccination strategies building on an ancestral viral strain need to be optimized for the control of potentially emerging viral variants. Therefore, aiming at strong B cell somatic hypermutation to increase antibody affinity to the ancestral strain - not only at high antibody titers - is a priority when utilizing vaccines that are not targeted at individual variants since high affinity may offer some flexibility to compensate for strain-individual mutations. Here, we developed a next-generation sequencing based SARS-CoV-2 B cell tracking protocol to rapidly determine the level of immunoglobulin somatic hypermutation at distinct points during the immunization period. The percentage of somatically hypermutated B cells in the SARS-CoV-2 specific repertoire was low after the primary vaccination series, evolved further over months and increased steeply after boosting. The third vaccination mobilized not only naïve, but also antigen-experienced B cell clones into further rapid somatic hypermutation trajectories indicating increased affinity. Together, the strongly mutated post-booster repertoires and antibodies deriving from this may explain why the third, but not the primary vaccination series, offers some protection against immune-escape variants such as Omicron B.1.1.529. |
URI: | https://opendata.uni-halle.de//handle/1981185920/88296 http://dx.doi.org/10.25673/86343 |
Open-Access: | ![]() |
Nutzungslizenz: | ![]() |
Sponsor/Geldgeber: | Publikationsfonds MLU |
Journal Titel: | Frontiers in immunology |
Verlag: | Frontiers Media |
Verlagsort: | Lausanne |
Band: | 13 |
Originalveröffentlichung: | 10.3389/fimmu.2022.876306 |
Enthalten in den Sammlungen: | Open Access Publikationen der MLU |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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fimmu-13-876306.pdf | 7.25 MB | Adobe PDF | ![]() Öffnen/Anzeigen |